Decreased progranulin expression in Mycosis fungoides: a possible association with the high frequency of skin infections
Decreased progranulin expression in Mycosis fungoides: a possible association with the high frequency of skin infections
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DOI：
10.1684/ejd.2018.3452
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发表时间：
2018-11-01
影响因子：
2.5
通讯作者：
Sato, Shinichi
中科院分区：
文献类型：
--
作者：
Nakajima, Rina;Miyagaki, Tomomitsu;Sato, Shinichi
背景：前颗粒蛋白（PGRN）是一种已知参与多种生物过程的多功能蛋白，包括肿瘤发生、抗炎症和抗感染。在多种恶性肿瘤中，血清或组织中的PGRN表达升高，并与不良预后相关。然而，PGRN是否参与蕈样真菌病（Mycosis fungoides， MF）仍有待确定。目的：探讨PGRN在MF中的作用。材料和方法：采用酶联免疫吸附法测定MF患者和正常人血清PGRN水平。采用定量逆转录聚合酶链反应和免疫组织化学检测MF和正常皮肤中PGRN的表达。此外，我们分析了病变皮肤中PGRN和抗菌肽表达水平之间的相关性。结果：MF患者血清PGRN水平明显低于正常对照组。免疫组化染色显示，正常对照表皮角质形成细胞表达PGRN，但MF皮肤表皮角质形成细胞表达PGRN也较弱。此外，PGRN与抗菌肽mRNA表达呈显著负相关。结论：PGRN低表达可能与MF患者皮肤感染的多发有关。
Background: Progranulin (PGRN) is a multi-functional protein known to be involved in diverse biological processes, including tumourigenesis, anti-inflammation, and anti-infection. PGRN expression in sera or tissues is elevated in a variety of malignancies and is associated with poor prognosis. However, it remains to be determined whether PGRN is involved in Mycosis fungoides (MF). Objectives: To investigate the roles of PGRN in MF. Materials and methods: Serum PGRN levels were measured in patients with MF and normal controls by enzyme-linked immunosorbent assay. PGRN expression in MF and normal skin was examined by quantitative reverse transcription polymerase chain reaction and immunohistochemistry. Moreover, we analysed correlations between the expression levels of PGRN and antimicrobial peptides in lesional skin. Results: PGRN levels were significantly lower in sera of MF patients than those of normal controls. PGRN mRNA levels in lesional skin of MF were also significantly decreased Immunohistochemical staining revealed that PGRN was expressed in epidermal keratinocytes of normal controls, however, PGRN expression in epidermal keratinocytes was also weaker in MF skin. Furthermore, significant inverse correlations were identified between PGRN and antimicrobial peptide mRNA expression. Conclusion: These results suggest that low PGRN expression may contribute to the frequent occurrence of skin infections in patients with MF.