High-speed volumetric imaging of cone photoreceptors with adaptive optics spectral-domain optical coherence tomography
High-speed volumetric imaging of cone photoreceptors with adaptive optics spectral-domain optical coherence tomography
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DOI：
10.1364/oe.14.004380
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发表时间：
2006-05-15
期刊：
影响因子：
3.8
通讯作者：
Miller, Donald T.
中科院分区：
文献类型：
--
作者：
Zhang, Yan;Cense, Barry;Miller, Donald T.
我们报告了对活体人类视网膜中锥体光感受器的三维形态的首次观察。使用高速自适应光学 (AO) 谱域光学相干断层扫描 (SD-OCT) 相机采集图像。 AO 系统由 Shack-Hartmann 波前传感器和双压电晶片镜 (AOptix) 组成，以 12 Hz 的闭环速率测量和校正眼睛像差和系统像差。双压电晶片镜位于 XY 机械扫描仪和受试者眼睛之间。 SD-OCT 系统由一个超发光二极管和一个每秒采集 75,000 次 A 扫描的 512 像素行扫描电荷耦合器件 (CCD) 组成。这个速度比之前报道的速度快两倍多。通过快速采集带或不带 AO 补偿的视网膜小体积图像，可以最大程度地减少视网膜运动伪影。相机灵敏度足以检测来自所有主要视网膜层的反射。发现在内段/外段 (IS/OS) 交界处和 OS 后尖处的 C 扫描中观察到的亮点的规则分布彼此之间以及与预期的视锥细胞间距高度相关。未发现 OS 后尖与检查的其他视网膜层（包括视网膜色素上皮）之间存在相关性。 (c) 2006 年美国光学学会。
We report the first observations of the three-dimensional morphology of cone photoreceptors in the living human retina. Images were acquired with a high-speed adaptive optics (AO) spectral-domain optical coherence tomography (SD-OCT) camera. The AO system consisted of a Shack-Hartmann wavefront sensor and bimorph mirror (AOptix) that measured and corrected the ocular and system aberrations at a closed-loop rate of 12 Hz. The bimorph mirror was positioned between the XY mechanical scanners and the subject's eye. The SD- OCT system consisted of a superluminescent diode and a 512 pixel line scan charge-coupled device (CCD) that acquired 75,000 A-scans/s. This rate is more than two times faster than that previously reported. Retinal motion artifacts were minimized by quickly acquiring small volume images of the retina with and without AO compensation. Camera sensitivity was sufficient to detect reflections from all major retinal layers. The regular distribution of bright spots observed within C-scans at the inner segment/outer segment (IS/OS) junctions and at the posterior tips of the OS were found to be highly correlated with one another and with the expected cone spacing. No correlation was found between the posterior tips of the OS and the other retinal layers examined, including the retinal pigment epithelium. (c) 2006 Optical Society of America.