The mitochondrial genome of Dastarcus helophoroides (Coleoptera: Bothrideridae) and related phylogenetic analyses.
The mitochondrial genome of Dastarcus helophoroides (Coleoptera: Bothrideridae) and related phylogenetic analyses.
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DOI：
10.1016/j.gene.2014.12.026
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发表时间：
2015-04
期刊：
影响因子：
3.5
通讯作者：
Zhengqing Zhang;Xiaoji Wang;Rongzhou Li;Ruijian Guo;Wei Zhang;Wang Song;Chunfeng Hao;Huapeng Wang;Menglou Li
中科院分区：
文献类型：
--
作者：
Zhengqing Zhang;Xiaoji Wang;Rongzhou Li;Ruijian Guo;Wei Zhang;Wang Song;Chunfeng Hao;Huapeng Wang;Menglou Li
花绒寄甲（Dastarcushelophoroides）线粒体基因组全长15，878 bp（GenBank：KF811054.1），包括13个PCG、22个tRNA基因、2个rRNA基因和1个非编码区（D-loop）。基因组具有典型的基因顺序，与其他鞘翅目物种相同。除COI基因一般以非规范起始密码子起始外，所有蛋白质编码基因均以ATN密码子起始，终止于TA（A）或TAG。rrnL和rrnS的二级结构分别由48个螺旋（包含4个新提出的螺旋）和35个螺旋（包含2个新提出的螺旋）组成。除trnS 1（AGN）的二氢尿嘧啶臂（DHU臂）不能形成稳定的茎环结构外，其余22个tRNA均折叠成典型的三叶草二级结构。利用现有的29个物种的13个蛋白质编码基因（核苷酸数据集和核酸数据集）来推断这些目之间的系统发育关系。拟步甲总科（Tenebrionoidea）和黄瓜总科（Cucujoidea）为姐妹群，D.类蛇蜥属（Helophoroidesis），属于Cucujoidea（Bothrideridae）。本研究首先对D. helophoroidesmitogenome，和结果强烈支持目前的形态学为基础的假设。
The complete mitochondrial genome ofDastarcus helophoroides(Coleoptera: Bothrideridae) which consists of 13 PCGs, 22 tRNA genes, two rRNA genes and a non-coding region (D-loop), is sequenced for its nucleotide sequence of 15,878 bp (GenBank: KF811054.1). The genome has a typical gene order which is identical to other Coleoptera species. Except for COI gene generally starts with non-canonical initial codon, all protein-coding genes start with ATN codon and terminate with the stop codon TA(A) or TAG. The secondary structure of rrnL and rrnS consists of 48 helices (contains four newly proposed helices) and 35 helices (contains two newly proposed helices) respectively. All 22 tRNAs inD. helophoroidesare predicted to fold into typical cloverleaf secondary structure, excepttrnS1(AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. Thirteen protein-coding genes (nucleotide dataset and nucleic acid dataset) of the available species (29 taxa) have been used to infer the phylogenetic relationships among these orders. Tenebrionoidea and Cucujoidea form a sister group, andD. helophoroidesis classified into Cucujoidea (Bothrideridae). The study first research on the phylogenetic analyses involving to theD. helophoroidesmitogenome, and the results strongly bolster the current morphology-based hypothesis.