A flow rate independent cell concentration measurement chip using electrical cell counters across a fixed control volume
A flow rate independent cell concentration measurement chip using electrical cell counters across a fixed control volume
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DOI：
10.1109/jmems.2007.906766
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发表时间：
2008-02-01
影响因子：
2.7
通讯作者：
Cho, Young-Ho
中科院分区：
文献类型：
--
作者：
Lee, Dong Woo;Yi, Soyeon;Cho, Young-Ho
我们提出了一种新的方法，测量细胞浓度使用两个电细胞计数器跨越一个固定的控制体积。我们的设备在固定控制体积的入口和出口对细胞进行计数，然后通过计算固定控制体积中的细胞数量来测量细胞浓度。以前测量细胞浓度的方法（如库尔特计数器和流式细胞仪）都试图在给定的液体体积或已知的流速下计数细胞。因此，尽管以前的设备是微型的，但其结果的准确性取决于外部机构的性能，如精密泵和流量传感器。然而，我们的原型并不依赖于精确的流体测量或流量的精确控制，因为它测量的是固定控制体积中的细胞数量。在实验研究中，我们在不测量或控制流速的情况下测量了5.8 × 10(5)至11.5 × 10(5)个细胞/mL的细胞浓度。对于细胞浓度的测量，我们的样机显示最大细胞浓度测量误差为10.3%，在血球计的误差范围内。
We present a novel method of measuring cell concentration using two electrical cell counters across a fixed control volume. Our device counts cells at the inlet and outlet of a fixed control volume and then measures the cell concentration by calculating the number of cells in the fixed control volume. Previous methods of measuring cell concentration (such as a Coulter counter and a flow cytometer) have attempted to count cells in a given fluid volume or at a known flow rate. Thus, in spite of the miniature nature of previous devices, the accuracy of their results depends on the performance of external mechanisms such as delicate pumps and flow sensors. Our prototype, however, does not depend on accurate fluid measurement or precise control of the flow rate because it measures the number of cells in a fixed control volume. In the experimental study, we measured cell concentrations ranging from 5.8 x 10(5) to 11.5 x 10(5) cells/mL without measuring or controlling the flow rate. For measuring the cell concentration, our prototype shows a maximum cell concentration measurement error of 10.3%, which is within the error range of a hemacytometer.