Effects of locally targeted heavy-ion and laser microbeam on root hydrotropism in Arabidopsis thaliana
Effects of locally targeted heavy-ion and laser microbeam on root hydrotropism in Arabidopsis thaliana
复制标题
DOI：
10.1269/jrr.07131
复制
发表时间：
2008-07-01
影响因子：
2
通讯作者：
Takahashi, Hideyuki
中科院分区：
文献类型：
--
作者：
Miyazawa, Yutaka;Sakashita, Tetsuya;Takahashi, Hideyuki
拟南芥/重离子微束/根向水性/激光消融/放射外科。经典的根系向水性研究都假设了根柱细胞的重要性以及从头基因表达的重要性，如生长素诱导基因。在向水性中的伸长区;然而，没有证实伸长区中的柱状细胞或生长素介导的信号传导对于向水性是必需的。利用重离子和激光微束技术研究了根冠和伸长区细胞在根向水性中的作用。延伸区的重离子微束辐照。但对柱细胞的作用不明显，能暂时抑制向水弯曲的发育。然而，激光消融证实，小柱细胞是必不可少的向水性。全身重离子宽束照射抑制吲哚乙酸5基因的从头表达。而不是MIZU-KUSSEI 1基因。我们的研究结果表明，根冠和伸长区有不可或缺的和功能上不同的作用，在根的向水性，和从头基因表达可能需要在伸长区的向水性，但不是在柱细胞。
Arabidopsis thaliana/Heavy-ion microbeam/Root hydrotropism/Laser ablation/Radiosurgery. Classical studies on root hydrotropism have hypothesized the importance Of columella Cells as well as the de novo gene expression, such as auxin-inducible gene. at the elongation zone in hydrotropism; however, there has been no confirmation that columella cells or auxin-mediated signaling in the elongation zone are necessary for hydrotropism. We examined the role of root cap and elongation zone cells in root hydrotropism using heavy-ion and laser microbeam. Heavy-ion microbeam irradiation of the elongation zone. but not that of the columella cells, significantly and temporarily Suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy-ion broad-beam irradiation suppressed de novo expression of INDOLE ACETIC ACID 5 gene. but not MIZU-KUSSEI1 gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that de novo gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.