Orientia tsutsugamushi suppresses the production of inflammatory cytokines induced by its own heat-stable component in murine macrophages
Orientia tsutsugamushi suppresses the production of inflammatory cytokines induced by its own heat-stable component in murine macrophages
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DOI：
10.1006/mpat.2001.0457
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发表时间：
2001-09-01
影响因子：
3.8
通讯作者：
Kang, JS
中科院分区：
文献类型：
--
作者：
Kim, MK;Kang, JS
恙虫病东方体是引起恙虫病的革兰氏阴性专性胞内细菌。为了了解恙虫病的发病机制，我们研究了O.两种鼠巨噬细胞系中恙虫病的研究活的和热灭活的orientia都刺激J774A.1细胞中细胞因子的产生。多粘菌素B不影响细胞因子的分泌。这些与未成熟的巨噬细胞系P388D1在被活的或热灭活的O.恙虫病强烈反对脂多糖（LPS）在细胞因子产生中的任何作用。热灭活O.与活的生物体相比，恙虫病的热稳定性强有力地表明，热稳定分子可能负责诱导细胞因子的产生和O。恙虫病可能具有抑制自身热稳定分子诱导的炎性细胞因子产生的机制。(C)北京：科学出版社.
Orientia tsutsugamushi is a Gram-negative obligate intracellular bacterium, which causes scrub typhus. To understand the pathogenesis of scrub typhus, we have investigated the induction of tumor necrosis alpha (TNF-alpha) and interleukin-6 (IL-6) by O. tsutsugamushi in two murine macrophage cell lines. Both live and heat-killed orientia stimulated the production of cytokines in J774A.1 cells. Polymyxin B does not affect the secretion of cytokines. These together with the fact that the immature macrophage cell line, P388D1, did not produce,TNF-alpha when induced by either live or heat-killed O. tsutsugamushi strongly argue against any roles of lipopolysaccharide (LPS) in cytokine production. Furthermore, the result that the cytokine responses were more brisk when macrophage cell lines had been induced by heat-killed O. tsutsugamushi than by live organisms strongly suggest that a heat-stable molecule might be responsible for the induction of cytokine production and O. tsutsugamushi might have mechanisms suppressing the production of inflammatory cytokines induced by its own heat-stable molecule. (C) 2001 Academic Press.