Insulin-like growth factor I slows the rate of denervation induced skeletal muscle atrophy
Insulin-like growth factor I slows the rate of denervation induced skeletal muscle atrophy
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DOI：
10.1016/j.nmd.2004.10.013
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发表时间：
2005-02-01
影响因子：
2.8
通讯作者：
Grounds, MD
中科院分区：
文献类型：
--
作者：
Shavlakadze, T;White, JD;Grounds, MD
骨骼肌神经供应的丧失导致肌肉质量随着时间的推移而无情地丧失（萎缩）。在转基因MLC/mIGF-1小鼠（其在分化的肌纤维中特异性过表达mIGF-1）中切断坐骨神经后，检查胰岛素样生长因子-1减少去神经引起的萎缩的能力。在去神经支配后28天，在转基因和野生型小鼠的胫骨前肌中测量所有类型的肌纤维和特别是IIB型肌纤维的横截面积（CSA）。在这段时间内，野生型小鼠的肌肉中有明显的肌纤维萎缩（类似于60%），具有小CSA的肌纤维数量增加。在MLC/mIGF-1小鼠的肌肉中，与野生型肌肉相比，mIGF-1的过表达使去神经诱导的肌纤维萎缩率降低了约30%，并保留了具有较大CSA的肌纤维。有人提出，mIGF-1对失神经肌纤维的保护作用可能是由于减少蛋白质分解。(C)2004 Elsevier B. V.保留所有权利。
Loss of the nerve supply to skeletal muscle results in a relentless loss of muscle mass (atrophy) over time. The ability of insulin-like growth factor-1 to reduce atrophy resulting from denervation was examined after transection of the sciatic nerve in transgenic MLC/mIGF-1 mice that over-express mIGF-1 specifically in differentiated myofibres. The cross sectional area (CSA) of all types of myofibres and specifically type IIB myofibres was measured in tibialis anterior muscles from transgenic and wild-type mice at 28 days after denervation. There was a marked myofibre atrophy (similar to60%) in the muscles of wild-type mice over this time with increased numbers of myofibres with small CSA. In the muscles of MLC/mIGF-1 mice, over-expression of mIGF-1 reduced the rate of denervation induced myofibre atrophy by similar to30% and preserved myofibres with larger CSA, compared to wild-type muscles. It is proposed that the protective effect of mIGF-1 on denervated myofibres might be due to reduced protein breakdown. (C) 2004 Elsevier B.V. All rights reserved.