The mucilage of yam (Dioscorea batatas Decne) tuber exhibited angiotensin converting enzyme inhibitory activities
The mucilage of yam (Dioscorea batatas Decne) tuber exhibited angiotensin converting enzyme inhibitory activities
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山药 (Dioscorea batatas Decne) 块茎的粘液表现出血管紧张素转换酶抑制活性
DOI：
10.7016/bbas.200310.0267
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
W. Hou
中科院分区：
文献类型：
--
作者：
Mei;Yin;Yaw‐Huei Lin;F. Hsu;W. Hou
山药块茎粘液经2-巯基乙醇处理后，经甲苯胺蓝染色后，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中显示分子量大于250 kDa的单一条带。使用（N-（3-[2-呋喃基]丙烯酰基）-Phe-Gly-Gly）（FAPGG）作为底物，通过分光光度法显示纯化的YTM以剂量依赖性方式抑制血管紧张素转换酶（ACE）（102.46 - 409.84 µg/mL YTM分别抑制28.7 - 59.8% ACE）。YTM抑制ACE活性50%所需的浓度（IC 50）为256.2 µg/mL，而卡托普利为0.00781 µM（0.0095 nmole）。商业多糖果胶（102.46至307.38 µg/mL）对ACE无抑制活性。用荧光硅胶薄层色谱法和C18反相高效液相色谱法检测FAPGG和FAP，结果也表明YTM对ACE有抑制作用。YTM对ACE呈混合型抑制作用，米氏常数为0.33mM。YTM对ACE表现出混合型抑制作用，其米氏常数为0.33 mM，食用山药可能有益于人们的健康。
The tuber mucilage of yam (Dioscorea batatas Decne) (YTM) was extracted and purified to homogeneity, which was confirmed by the toluidine blue staining on a sodium dodecylsulfate-polyacrylamide gel electrophoresis gel treated with 2-mercaptoethanol appearing as a single band with molecular mass larger than 250 kDa. This purified YTM was shown by spectrophotometric method to inhibit angiotensin converting enzyme (ACE) in a dose-dependent manner (28.7 to 59.8% ACE inhibition, respectively, by 102.46 to 409.84 µg/mL YTM) using (N-(3-[2-furyl]acryloyl)-P he-Gly-Gly) (FAPGG) as a substrate. The concentration of YTM required for 50% inhibition (IC50) of ACE activity was 256.2 µg/mL while that of captopril was 0.00781 µM (0.0095 nmole). The commercial polysaccharide pectin (102.46 to 307.38 µg/mL) showed no inhibitory activity against ACE. Using fluorescent silica TLC or C18 reverse phase HPLC to detect FAPGG and FAP, the results also showed that YTM inhibited ACE. The YTM showed mixed type inhibition against ACE, and the Michaelis constant in the presence of YTM was 0.33 mM. Consumption of yam tubers may benefit people's health. The YTM showed mixed type inhibition against ACE, and the Michaelis constant in the presence of YTM was 0.33 mM. Consumption of yam tubers may benefit people's health.