The molecular epidemiology of HIV-1 among male sex workers in northern Thailand.
The molecular epidemiology of HIV-1 among male sex workers in northern Thailand.
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泰国北部男性性工作者中 HIV-1 的分子流行病学。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
C. Natpratan
中科院分区：
文献类型：
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作者：
Chris Beyrer;A. Artenstein;P. Kunawararak;Thomas C. VanCott;Carl J. Mason;Kittipong Rungreungthanakit;P. Hegerich;K. Nelson;Chirasak Khamboonruang;C. Natpratan
泰国北方的男性商业性工作者（CSW）感染HIV-1的风险很高，可能通过与来自泰国、其他亚洲国家和西方的男性和女性发生性接触而暴露于多种HIV-1亚型。我们研究了103名在清迈市同性恋酒吧和俱乐部工作的泰国男性国民，他们参加了1994年泰国公共卫生部的哨点监测，使用HIV血清学检测、DNA聚合酶链反应方法、分子克隆、差异菌落杂交、核苷酸测序、和第三高变区（V3）肽酶联免疫吸附测定，以区分感染HIV-1亚型B、E、两者或两者都不感染的受试者。大多数人（58%）报告异性恋取向，35人（34%）已婚。17例（16.5%）为HIV感染者：17例中16例（94%）为HIV-1 E亚型; 1例为HIV-1 B亚型。两名受试者通过分子技术得到双重反应结果，但菌落杂交测序仅显示HIV-1亚型E。HIV-1亚型E似乎是泰国北方男性CSW中的主要分支。这可能对艾滋病毒流行病的全球动力学产生重要影响。
Male commercial sex workers (CSWs) in northern Thailand are at high risk for HIV-1 infection and may be exposed to multiple subtypes of HIV-1 through sexual contact with men and women from Thailand, other Asian nations, and the West. We studied 103 male Thai nationals working in gay bars and clubs in Chiang Mai city who participated in the 1994 sentinel surveillance of the Thai Ministry of Public Health using HIV serologic testing, DNA polymerase chain reaction methods, molecular cloning, differential colony hybridization, nucleotide sequencing, and a third hypervariable region (V3) peptide enzyme-linked immunoabsorbent assay to differentiate subjects infected with HIV-1 subtypes B, E, both, or neither. The majority (58%) reported heterosexual orientation, and 35 (34%) were married. Seventeen (16.5%) were HIV infected: 16 (94%) of 17 had HIV-1 subtype E; 1 had HIV-1 subtype B. Two subjects had dually reactive results by molecular techniques but colony hybridization sequencing revealed only HIV-1 subtype E. HIV-1 subtype E appears to be the predominant clade among male CSWs in northern Thailand. This may have important implications for the global kinetics of the HIV epidemic.
抗体-gp120 结合相互作用的解离率可预测 V3 介导的 HIV-1 中和作用。
DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
VanCott,TC;Bethke,FR;Polonis,VR;Gorny,MK;Zolla-Pazner,S;Redfield,RR;Birx,DL
通讯作者：
Birx,DL