TaqMan based real time PCR for the quantification of canine distemper virus
TaqMan based real time PCR for the quantification of canine distemper virus
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DOI：
10.1007/s11259-007-0020-9
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发表时间：
2007-08-01
影响因子：
2.2
通讯作者：
Morganti, L.
中科院分区：
文献类型：
--
作者：
Scagliarini, A.;Dal Pozzo, F.;Morganti, L.
犬瘟热病毒是一种高致死率疾病的病原体，影响全世界的狗。该疾病引起可变的临床体征，使得临床诊断非常困难（阿佩尔和萨默斯，1995）。亚临床感染的犬有助于疾病的扩散，因此开发用于检测这些亚临床感染犬中病毒的极其敏感的诊断试验至关重要。目前还没有关于细胞培养物中CDV生长动力学的数据;这些数据对于评价不同化合物针对CDV的体外抗病毒活性是非常重要的（Scagliarini等人，2006年）。本研究的目的是建立一种快速、灵敏的真实的实时RT-PCR检测方法，用于临床标本和细胞培养物中CDV的检测和定量。
Canine distemper virus is the causative agent of a high lethality rate disease affecting dogs worldwide. The disease causes variable clinical signs that make clinical diagnosis very difficult (Appel and Summers, 1995). Sub clinically infected dogs contribute to the diffusion of the disease, therefore the development of an extremely sensitive diagnostic test for the detection of the virus in these subclinically infected dogs is of crucial importance. At present there are no data on CDV growth dynamics in cell culture; these data would be very important to evaluate the in vitro antiviral activity of different compounds against CDV (Scagliarini et al., 2006). The aim of this study was to develop a rapid and sensitive real time RT-PCR assay based on TaqMan technology to detect and quantify CDV in clinical samples and cell cultures.