The nucleotide sequence and genomic organization of Citrus leaf blotch virus: Candidate type species for a new virus genus
The nucleotide sequence and genomic organization of Citrus leaf blotch virus: Candidate type species for a new virus genus
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DOI：
10.1006/viro.2001.1040
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发表时间：
2001-08-15
期刊：
影响因子：
3.7
通讯作者：
Guerri, J
中科院分区：
文献类型：
--
作者：
Vives, MC;Galipienso, L;Guerri, J
测定了柑橘叶斑病病毒(CLBV)的全序列。CLBV基因组RNA(GRNA)全长8747个核苷酸，5‘端和3’端分别含有73和541个核苷酸的开放阅读框(ORF)和非翻译区(UTR)。ORF1编码一个227.4 kDa的多肽，具有甲基转移酶、类木瓜蛋白酶、解旋酶和依赖核糖核酸的核糖核酸聚合酶基序。ORF2编码一个40.2 kDa的多肽，含有细胞间运动蛋白的基序。ORF3编码的40.7 kDa多肽被鉴定为外壳蛋白。CLBV的基因组结构与毛状病毒属病毒相似，但在许多方面不同：(1)毛状病毒的ORF2与ORF1和ORF3重叠，而CLBV的ORF2和ORF3是分离的，ORF1和ORF2在一个核苷酸中重叠；(Ii)CLBV的gRNA和CP大于毛状病毒的gRNA和CP；(Iii)CLBV的3‘非编码区大于毛状病毒的。基于CP氨基酸特征的系统发育比较清楚地将CLBV与毛状病毒区分开来。与毛状病毒相比，CLBV也不能传播到藜属植物上。考虑到这些奇异性，我们建议将CLBV归入一个新的病毒属。(C)2001年学术出版社。
The complete nucleotide sequence of Citrus leaf blotch virus (CLBV) was determined. CLBV genomic RNA (gRNA) has 8747 nt, excluding the 3'-terminal poly(A) tail, and contains three open reading frames (ORFs) and untranslated regions (UTR) of 73 and 541 nucleotides at the 5' and 3' termini, respectively. ORF1 potentially encodes a 227.4-kDa polypeptide, which has methyltransferase, papain-like protease, helicase, and RNA-dependent RNA polymerase motifs. ORF2 encodes a 40.2-kDa polypeptide containing a motif characteristic of cell-to-cell movement proteins. The 40.7-kDa polypeptide encoded by ORF3 was identified as the coat protein. The genome organization of CLBV resembles that of viruses in the genus Trichovirus, but they differ in various aspects: (i) in trichoviruses ORF2 overlaps ORFs 1 and 3, whereas in CLBV, ORFs 2 and 3 are separated and ORFs 1 and 2 overlap in one nucleotide; (ii) CLBV gRNA and CP are larger than those of trichoviruses; and (iii) the CLBV 3' UTR is larger than that of trichoviruses. Phylogenetic comparisons based on CP amino acid signatures clearly separates CLBV from trichoviruses. Also contrasting with trichoviruses, CLBV could not be transmitted to Chenopodium quince Willd. Considering these singularities, we propose that CLBV should be included in a new virus genus. (C) 2001 Academic Press.