Neisseria gonorrhoeae catalase is not required for experimental genital tract infection despite the induction of a localized neutrophil response.
Neisseria gonorrhoeae catalase is not required for experimental genital tract infection despite the induction of a localized neutrophil response.
复制标题
尽管诱导了局部中性粒细胞反应，但实验性生殖道感染不需要淋病奈瑟菌过氧化氢酶。
DOI：
10.1128/iai.01513-06
复制
发表时间：
2007
影响因子：
3.1
通讯作者：
Jerse,AnnE
中科院分区：
文献类型：
--
作者：
Soler-Garcia,AngelA;Jerse,AnnE
淋病奈瑟菌产生几种抗氧化防御，包括高水平的过氧化氢酶，这可能有助于通过中和吞噬细胞产生的H2 O2在炎症反应期间持续存在。过氧化氢酶在淋球菌存活中的作用的体内测试是至关重要的，因为几个生理因素影响N。淋病和多形核白细胞（PMNs）。在这里，我们评估淋球菌过氧化氢酶在女性生殖道感染的替代模型的重要性。雌性BALB/c小鼠用17-β雌二醇处理以促进对N的易感性。淋病并阴道内接种野生型淋球菌或过氧化氢酶（KAT）缺失突变体。分别有43%和81%的野生型orkat突变淋球菌感染小鼠出现局部PMN流入，在阴道涂片中观察到与许多野生型或过氧化氢酶缺陷型细菌相关的PMN。六个实验的组合结果显示，与过氧化氢酶缺陷型淋球菌相比，野生型细菌恢复的天数存在显著差异。然而，实验之间存在很大的变异性，我们发现PMN流入，定植负荷和野生型orkat突变细菌的清除之间没有相关性。雌二醇治疗并不损害细菌的摄取，鲁米诺依赖的化学发光反应，或杀死能力的分离的小鼠中性粒细胞againstN。淋病或金黄色葡萄球菌。我们的数据是不可靠的。淋球菌不受小鼠下生殖道中性粒细胞产生的H2 O2的显著攻击;或者，多余的防御机制可能在感染过程中保护淋球菌免受活性氧的侵害。
Neisseria gonorrhoeaeproduces several antioxidant defenses, including high levels of catalase, which may facilitate the persistence during an inflammatory response via neutralization of H2O2produced by phagocytes. In vivo testing of the role of catalase in gonococcal survival is critical since several physiological factors impact interactions betweenN. gonorrhoeaeand polymorphonuclear leukocytes (PMNs). Here we assessed the importance of gonococcal catalase in a surrogate model of female genital tract infection. Female BALB/c mice were treated with 17-β estradiol to promote susceptibility toN. gonorrhoeaeand inoculated intravaginally with wild-type gonococci or a catalase (kat) deletion mutant. A localized PMN influx occurred in an average of 43 and 81% of mice infected with wild-type orkatmutant gonococci, respectively, and PMNs associated with numerous wild-type or catalase-deficient bacteria were observed in vaginal smears. The combined results of six experiments showed a significant difference in the number of days wild-type bacteria were recovered compared to the catalase-deficient gonococci. However, there was much variability between experiments, and we found no correlation between PMN influx, colonization load, and clearance of wild-type orkatmutant bacteria. Estradiol treatment did not impair bacterial uptake, the luminol-dependent chemiluminescence response, or the killing capacity of isolated murine PMNs againstN. gonorrhoeaeorStaphylococcus aureus. Our data suggestN. gonorrhoeaeis not significantly challenged by H2O2produced by PMNs in the murine lower genital tract; alternatively, redundant defense mechanisms may protect the gonococcus from reactive oxygen species during infection.