Isolation of sublineage I Nitrospira by a novel cultivation strategy
Isolation of sublineage I Nitrospira by a novel cultivation strategy
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DOI：
10.1111/1462-2920.12248
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发表时间：
2014-10-01
影响因子：
5.1
通讯作者：
Aoi, Yoshiteru
中科院分区：
文献类型：
--
作者：
Fujitani, Hirotsugu;Ushiki, Norisuke;Aoi, Yoshiteru
硝化作用是生物地球化学氮循环中的一个重要过程，在废水的生物处理中得到了广泛的应用。近年来，硝化螺旋菌已被公认为亚硝酸盐氧化细菌属中数量占主导地位的一种，主要负责好氧硝化的第二步。然而，硝基螺旋菌的生理特性仍然知之甚少，因为这种生物很难分离和培养。在这里，我们报告了一种在纯培养中获得硝基螺旋藻亚系I成员的新培养策略。该方法结合：(i)利用连续进料反应器对硝化螺旋菌进行选择性富集；（ii）通过细胞分选系统对硝化螺旋菌进行纯化，然后进行次培养，重点关注形成球形微菌落的独特特征。该策略可能适用于其他未培养或未分离的硝基螺旋藻，并可能加速对这一重要生物群体的生理和生化理解。
Nitrification is an important process in the biogeochemical nitrogen cycle and is widely exploited in biological wastewater treatment. Recently, Nitrospira has been recognized as the numerically dominant nitrite-oxidizing bacterial genus and is primarily responsible for the second step of aerobic nitrification. Nevertheless, the physiological properties of Nitrospira remain poorly understood because the organisms are difficult to isolate and culture. Here, we report a novel cultivation strategy for obtaining members of the Nitrospira sublineage I in pure culture. The method combines: (i) selective enrichment of Nitrospira using a continuous feeding reactor and (ii) purification followed by sub-cultivation via a cell sorting system by focusing on the unique characteristics of Nitrospira forming spherical micro-colonies. This strategy is potentially applicable to other uncultured or unisolated Nitrospira and could accelerate the physiological and biochemical understandings of this important group of organisms.