Cutting edge: Ectopic expression of the chemokine TCA4/SLC is sufficient to trigger lymphoid neogenesis
Cutting edge: Ectopic expression of the chemokine TCA4/SLC is sufficient to trigger lymphoid neogenesis
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DOI：
10.4049/jimmunol.164.8.3955
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发表时间：
2000-04-15
影响因子：
4.4
通讯作者：
Lo, D
中科院分区：
文献类型：
--
作者：
Fan, L;Reilly, CR;Lo, D
为了测试幼稚淋巴细胞的积累是否足以触发淋巴发育，我们产生了具有胰岛表达的趋化因子TCA 4/SLC的小鼠。该趋化因子对幼稚淋巴细胞和表达CCR 7受体的成熟树突状细胞（DC）具有特异性。胰岛最初发展为T细胞与DC的积聚，在周边具有分散的B细胞。浸润淋巴细胞表现为幼稚型CD 14（低）CD 25（-）CD 69（-）表型，但半数为CD 62 L阴性。当回交至RAG-1敲除时，不招募DC。有趣的是，尽管没有正常的外周淋巴结，但在与Ikaros基因敲除小鼠的回交中仍发育出了胰岛淋巴组织。我们的研究结果表明，TCA 4/SLC可以通过不同的募集T和B淋巴细胞以及对基质细胞发育的次级作用来诱导淋巴组织的发育和组织化。
To test whether accumulation of naive lymphocytes is sufficient to trigger lymphoid development, we generated mice with islet expression of the chemokine TCA4/SLC, This chemokine is specific for naive lymphocytes and mature dendritic cells (DC) which express the CCR7 receptor. Islets initially developed accumulations of T cells with DC, with scattered B cells at the perimeter. These infiltrates consolidated into organized lymphoid tissue, with high endothelial venules and stromal reticulum, Infiltrate lymphocytes showed a naive CD14(low) CD25(-) CD69(-) phenotype, though half were CD62L negative. When backcrossed to RAG-1 knockout, DC were not recruited. Interestingly, islet lymphoid tissue developed in backcrosses to Ikaros knockout mice despite the absence of normal peripheral nodes. Our results indicate that TCA4/SLC can induce the development and organization of lymphoid tissue through diffential recruitment of T and B lymphocytes and secondary effects on stromal cell development.