Floral nectar phenolics as biochemical markers for the botanical origin of heather honey
Floral nectar phenolics as biochemical markers for the botanical origin of heather honey
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DOI：
10.1007/bf01229682
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发表时间：
1996-01-01
期刊：
影响因子：
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通讯作者：
TomasBarberan, FA
中科院分区：
文献类型：
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作者：
Ferreres, F;Andrade, P;TomasBarberan, FA
为了找到石南花蜂蜜的植物来源的生化标记，对从石南花采集花蜜的蜜蜂的蜜胃中收集的石南花花蜜中存在的酚类代谢物进行了分析。花蜜的类黄酮部分含有四种主要的类黄酮。其中2种为槲皮素和山奈酚3-鼠李糖苷，另外2种初步鉴定为杨梅素3'-甲基醚和异鼠李糖素3-鼠李糖苷。由于天然糖苷被蜜蜂酶水解生成相应的苷元，这是蜂蜜中检测到的代谢物，因此实现了花蜜糖苷的酸水解。鉴定出槲皮素、杨梅素3′-甲基醚、山奈酚和异鼠李素。以及没食子酸的衍生物鞣花酸。对葡萄牙石楠花蜂蜜样品的分析表明，所有样品中都含有鞣花酸，含量在每100克蜂蜜100至600毫克之间。其他从花蜜中提取的类黄酮也存在，尽管其中一些含量变化很大。鞣花酸和杨梅素3′-甲基醚，迄今为止尚未在任何一种单花蜂蜜样品中检测到，唯一的例外是一种与植物相关的法国蜂蜜样品，其中也含有鞣花酸，似乎是最有用的潜在标记石南花蜂蜜的花源。然而，需要更详细和广泛的调查来证明这些标记的效用。
In order to find out biochemical markers for the botanical origin of heather (EI ica) honey, the phenolic metabolites present in heather floral nectar: collected from the honey-stomach of bees gathering nectar from these flowers, were analysed. The flavonoid fraction of nectar contained four main flavonoids. Two of them were quercetin and kaempferol 3-rhamnosides, and the other two were tentatively identified as myricetin 3'-methyl ether and isorhamnetin 3-rhamnosides. Since the natural glycosides are hydrolysed by bee enzymes to render the corresponding aglycones, which are the metabolites detected in honey, acid hydrolysis of the nectar glycosides was achieved. The aglycones quercetin, myricetin 3'-methyl ether, kaempferol and isorhamnetin were identified. as well as the gallic acid derivative ellagic acid. The analysis of Portuguese heather honey samples showed that ellagic acid was present in all the samples in significant amounts ranging between 100 mu g and 600 mu g per 100 g honey. The other nectar-derived flavonoids were also present, although some of them in very variable amounts. Ellagic acid and myricetin 3'-methyl ether, which have not been detected in any of the monofloral honey samples investigated so far, with the only exception being a French honey sample of the botanically related Calluna (Ericaceae) which also contained ellagic acid, seem to be the most useful potential markers for the floral origin of heather honey. However, more detailed and extensive investigations are needed to prove the utility of these markers.