Analyzing the dynamic bacterial glycome with a lectin microarray approach
Analyzing the dynamic bacterial glycome with a lectin microarray approach
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DOI：
10.1038/nchembio767
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发表时间：
2006-03-01
影响因子：
14.8
通讯作者：
Mahal, LK
中科院分区：
文献类型：
--
作者：
Hsu, KL;Pilobello, KT;Mahal, LK
细菌细胞表面的糖基化正在成为共生、发病机制、细胞-细胞相互作用和免疫逃避中的关键因素（1-3）。缺乏高通量分析工具来检查细菌聚糖一直是该领域的主要障碍，并阻碍了对碳水化合物变化动力学的更深入研究。我们最近开发了一种用于糖蛋白分析的凝集素微阵列（4）。在此，我们提出了一个快速分析系统的基础上，这一技术的检查细菌聚糖。观察到的糖基化模式将密切相关的大肠杆菌菌株彼此区分开来，提供了一种简单的细菌指纹识别方法。此外，致病性大肠杆菌碳水化合物外壳的动态变化。大肠杆菌菌株容易观察到。快速评估表面碳水化合物表位的实时变化，可以检查细菌糖在响应外部刺激（如免疫系统）时的动态作用。
Glycosylation of bacterial cell surfaces is emerging as a critical factor in symbiosis, pathogenesis, cell-cell interactions and immune evasion(1-3). The lack of high-throughput analytical tools to examine bacterial glycans has been a major obstacle to the field and has hindered closer examination of the dynamics of carbohydrate variation. We have recently developed a lectin microarray for the analysis of glycoproteins(4). Herein we present a rapid analytical system based on this technology for the examination of bacterial glycans. The glycosylation pattern observed distinguishes closely related Escherichia coli strains from one another, providing a facile means of fingerprinting bacteria. In addition, dynamic alterations in the carbohydrate coat of a pathogenic E. coli strain are readily observed. The fast evaluation of real-time alterations in surface-carbohydrate epitopes allows examination of the dynamic role of bacterial sugars in response to external stimuli such as the immune system.