Content of nonhistone protein in nuclei after hyperthermic treatment.
Content of nonhistone protein in nuclei after hyperthermic treatment.
复制标题
热疗后细胞核中非组蛋白的含量。
DOI：
10.1002/jcp.1041540202
复制
发表时间：
1993
影响因子：
5.6
通讯作者：
Dewey,WC
中科院分区：
文献类型：
--
作者：
Chu,GL;Ross,G;Wong,RS;Warters,R;Dewey,WC
当从加热后的中国仓鼠卵巢细胞中分离细胞核时，相对于整个细胞，细胞核中3 H-色氨酸标记的非组蛋白的量大幅增加。在45.5°C加热15 min或30 min后，核非组蛋白含量分别增加1.6或1.8。相反，当用放射自显影法定量固定细胞切片中细胞核和细胞质中的3 H-色氨酸标记蛋白质来测定完整细胞中的核非组蛋白质含量时，在45.5°C下15或30 min，核非组蛋白质含量分别仅增加1.14或1.28。因此，热不会诱导大量的细胞质蛋白进入细胞核。© 1993 Wiley利斯公司
When nuclei were isolated from Chinese hamster ovary cells after being heated, there was a large increase in the amount of3H‐tryptophan labeled nonhistone protein in the nucleusrelativeto the whole cell. After 15 min or 30 min of heating at 45.5°C, the nuclear nonhistone protein content increased by 1.6 or 1.8, respectively. In contrast, when the nuclear nonhistone protein content was determined in theintactcell by using autoradiography to quantify3H‐tryptophan labeled protein in the nucleus and cytoplasm in sections offixedcells, the nuclear nonhistone protein content increased by only 1.14 or 1.28 for 15 or 30 min at 45.5°C, respectively. Therefore, heat does not induce a massive movement of cytoplasmic protein into the nucleus. © 1993 Wiley‐Liss, Inc.