Genome-wide analysis of estrogen receptor binding sites
Genome-wide analysis of estrogen receptor binding sites
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DOI：
10.1038/ng1901
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发表时间：
2006-11-01
期刊：
影响因子：
30.8
通讯作者：
Brown, Myles
中科院分区：
文献类型：
--
作者：
Carroll, Jason S.;Meyer, Clifford A.;Brown, Myles
雌激素受体是乳腺癌表型的主要转录调节因子，也是分子治疗靶点的原型。我们绘制了乳腺癌细胞中全基因组范围内所有雌激素受体和RNA聚合酶II结合位点的图谱，确定了真正的顺式结合位点和靶基因。结合这一独特的资源与基因表达数据表明雌激素介导的基因调控，特别是在雌激素抑制基因的情况下，不同的时间机制。此外，这一资源已允许识别顺式调控位点在以前未开发的基因组区域和乳腺癌雌激素信号的基础上的合作转录因子。
The estrogen receptor is the master transcriptional regulator of breast cancer phenotype and the archetype of a molecular therapeutic target. We mapped all estrogen receptor and RNA polymerase II binding sites on a genome-wide scale, identifying the authentic cis binding sites and target genes, in breast cancer cells. Combining this unique resource with gene expression data demonstrates distinct temporal mechanisms of estrogen-mediated gene regulation, particularly in the case of estrogen-suppressed genes. Furthermore, this resource has allowed the identification of cis-regulatory sites in previously unexplored regions of the genome and the cooperating transcription factors underlying estrogen signaling in breast cancer.