A tentative mechanism of the ternary complex formation between phosphorylase kinase, glycogen phosphorylase b and glycogen
A tentative mechanism of the ternary complex formation between phosphorylase kinase, glycogen phosphorylase b and glycogen
复制标题
磷酸化酶激酶、糖原磷酸化酶b和糖原形成三元复合物的初步机制
DOI：
10.1016/s0014-5793(99)00125-8
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发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
N. B. Livanova
中科院分区：
文献类型：
--
作者：
I. E. Andreeva;V. F. Makeeva;B. Kurganov;N. A. Chebotareva;N. B. Livanova
本文研究了兔骨骼肌磷酸化酶激酶与糖原相互作用的动力学。在pH 6.8时，磷酸化酶激酶与糖原的结合仅在Mg 2+存在下进行，而在pH 8.2时，即使在不存在Mg 2+的情况下也会形成复合物。另一方面，磷酸化酶激酶与糖原的相互作用在两种pH值下都需要Ca2+。复合物形成的初始速率与酶和糖原的浓度成正比，表明在磷酸化酶激酶与糖原结合的初始步骤形成化学计量比为1：1的复合物。根据动力学和沉降数据，磷酸化酶激酶反应的底物糖原磷酸化酶b有利于磷酸化酶激酶与糖原的结合。我们提出了一个磷酸化酶B磷酸化酶激酶与糖原颗粒有序结合的模型，该模型解释了磷酸化酶B存在下磷酸化酶激酶与糖原结合的紧密性增加。
The kinetics of rabbit skeletal muscle phosphorylase kinase interaction with glycogen has been studied. At pH 6.8 the binding of phosphorylase kinase to glycogen proceeds only in the presence of Mg2+, whereas at pH 8.2 formation of the complex occurs even in the absence of Mg2+. On the other hand, the interaction of phosphorylase kinase with glycogen requires Ca2+at both pH values. The initial rate of the complex formation is proportional to the enzyme and glycogen concentrations, suggesting the formation of the complex with stoichiometry 1:1 at the initial step of phosphorylase kinase binding by glycogen. According to the kinetic and sedimentation data, the substrate of the phosphorylase kinase reaction, glycogen phosphorylaseb, favors the binding of phosphorylase kinase with glycogen. We suggest a model for the ordered binding of phosphorylaseband phosphorylase kinase to the glycogen particle that explains the increase in the tightness of phosphorylase kinase binding with glycogen in the presence of phosphorylaseb.
二价阳离子而非其他激活剂增强磷酸化酶激酶对糖原磷酸化酶的亲和力。
DOI：
10.1021/bi9528107
发表时间：
1996
期刊：
Biochemistry.
影响因子：
--
作者：
Xu,YH;Wilkinson,DA;Carlson,GM
通讯作者：
Carlson,GM
靠近α亚基羧基末端的表位位于磷酸化酶激酶十六聚体的叶尖附近。
影响因子：
5.6
作者：
Wilkinson,DA;Marion,TN;Tillman,DM;Norcum,MT;Hainfeld,JF;Seyer,JM;Carlson,GM
通讯作者：
Carlson,GM
影响因子：
5.6
作者：
Norcum,MT;Wilkinson,DA;Carlson,MC;Hainfeld,JF;Carlson,GM
通讯作者：
Carlson,GM