Tracking individual kinesin motors in living cells using single quantum-dot imaging
Tracking individual kinesin motors in living cells using single quantum-dot imaging
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DOI：
10.1021/nl060921t
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发表时间：
2006-07-12
期刊：
影响因子：
10.8
通讯作者：
Dahan, Maxime
中科院分区：
文献类型：
--
作者：
Courty, Sebastien;Luccardini, Camilla;Dahan, Maxime
我们报告了一种使用半导体量子点（QD）的简单方法，以高灵敏度跟踪细胞内蛋白质的运动。我们对活 HeLa 细胞中单个 QD 标记的驱动蛋白马达的体内运动进行了表征。单分子测量提供了电机的重要参数，例如其速度和加工能力，以及携带量子点所需的力的估计。我们的测量证明了单分子实验在细胞内运输研究中的重要性，以及单量子点成像在研究细胞运输、细胞极化和分裂等重要过程中的潜力。
We report a simple method using semiconductor quantum dots (QDs) to track the motion of intracellular proteins with a high sensitivity. We characterized the in vivo motion of individual QD-tagged kinesin motors in living HeLa cells. Single-molecule measurements provided important parameters of the motor, such as its velocity and processivity, as well as an estimate of the force necessary to carry a QD. Our measurements demonstrate the importance of single-molecule experiments in the investigation of intracellular transport as well as the potential of single quantum-dot imaging for the study of important processes such as cellular trafficking, cell polarization, and division.