Mass Spectrometry of Proteins - Methods and Protocols
Mass Spectrometry of Proteins - Methods and Protocols
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蛋白质质谱 - 方法和实验方案
DOI：
10.1007/978-1-4939-9232-4_10
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Russo D
中科院分区：
文献类型：
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作者：
Russo D
宏蛋白质组学的最新进展为我们提供了基因组表达与环境微生物群落功能表征之间的联系。因此，对环境微生物组表达的蛋白质进行大规模鉴定，可以前所未有地了解它们的原位代谢和功能。然而，宏蛋白质组学的主要挑战之一仍然是缺乏强大的分析管道。对于低蛋白质浓度的水生环境和已知会干扰传统样品制备管道和下游LC-MS/MS分析的化合物的存在尤其如此。在本章中，提供了一种从样品制备到功能注释的半定量方法。该方法已被证明可以提供淡水微生物组真核和原核部分的深入和代表性结果。
Recent advances in metaproteomics have provided us a link between genomic expression and functional characterization of environmental microbial communities. Therefore, the large-scale identification of proteins expressed by environmental microbiomes allows an unprecedented view of their in situ metabolism and function. However, one of the main challenges in metaproteomics remains the lack of robust analytical pipelines. This is especially true for aquatic environments with low protein concentrations and the presence of compounds that are known to interfere with traditional sample preparation pipelines and downstream LC-MS/MS analyses. In this chapter, a semiquantitative method that spans from sample preparation to functional annotation is provided. This method has been shown to provide in-depth and representative results of both the eukaryotic and prokaryotic fractions of freshwater microbiomes.