Combined measurement of ferritin, soluble transferrin receptor, retinol binding protein, and C-reactive protein by an inexpensive, sensitive, and simple sandwich enzyme-linked immunosorbent assay technique
Combined measurement of ferritin, soluble transferrin receptor, retinol binding protein, and C-reactive protein by an inexpensive, sensitive, and simple sandwich enzyme-linked immunosorbent assay technique
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DOI：
10.1093/jn/134.11.3127
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发表时间：
2004-11-01
影响因子：
4.2
通讯作者：
Craft, NE
中科院分区：
文献类型：
--
作者：
Erhardt, JG;Estes, JE;Craft, NE
维生素A（VA）和铁的测量是非常重要的营养缺乏的评估。本研究的目的是开发一种夹心ELISA技术，同时测量铁蛋白，可溶性转铁蛋白受体，视黄醇结合蛋白和C-反应蛋白（CRP）作为VA和铁状态的指标。CRP作为感染标志物的纳入允许更准确地解释VA和铁状态。这是在30-穆尔血清或血浆样品中使用ELISA用不同的捕获和检测抗体以及样品的不同稀释液完成的。市售临床血清质控品用于校准目的。将开发的检测方法与市售的传统检测方法进行了比较。两种方法的回归系数均优于0.84（P <0.001）。使用有限的样品集，夹心ELISA测定产生非常相似的特异性和灵敏度相比，传统的方法时，共同的截止值。所有检测的批内和批间变异性均在5 - 14%之间。如果分析大量样品，则用于所有5次测量的材料成本降低至低于$1/样品。由于成本低，高通量，与传统的测试和可比性，该程序具有几个优点，在人口调查中评估VA和铁的状态。
The measurement of vitamin A (VA) and iron status is very important in the assessment of nutritional deficiencies. The objective of this research was to develop a sandwich ELISA technique for the simultaneous measurement of ferritin, soluble transferrin receptor, retinol binding protein, and C-reactive protein (CRP) as indicators for VA and iron status. The inclusion of CRP as marker of infection allows for more accurate interpretation of VA and iron status. This is accomplished in a 30-muL serum or plasma sample using an ELISA with different capture and detection antibodies and different dilutions of the sample. Commercially available clinical serum controls were used for calibration purposes. The developed assays were compared to commercially available traditional tests. Regression coefficients comparing both assays were better than 0.84 (P < 0.001). Using a limited sample set, the sandwich ELISA assay produced very similar specificity and sensitivity compared to traditional methods when common cutoff values were applied. Intra- and interassay variability was between 5 and 14% for all tests. The cost of the materials for all 5 measurements decreases to less than $1/sample if a large number of samples is analyzed. Due to the low cost, high throughput, and comparability to traditional tests, this procedure has several advantages for assessing VA and iron status in population surveys.