Green synthesis of silver nanoparticles by Chrysanthemum morifolium Ramat. extract and their application in clinical ultrasound gel.
Green synthesis of silver nanoparticles by Chrysanthemum morifolium Ramat. extract and their application in clinical ultrasound gel.
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菊花拉马特绿色合成银纳米颗粒。
DOI：
10.2147/ijn.s43289
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发表时间：
2013
影响因子：
8
通讯作者：
Zhao F
中科院分区：
文献类型：
--
作者：
He Y;Du Z;Lv H;Jia Q;Tang Z;Zheng X;Zhang K;Zhao F
用植物提取物进行生态友好的绿色合成在纳米技术中起着非常重要的作用，没有任何有害的化学物质。本研究以杭白菊为原料，通过处理银离子，制备水溶性银纳米粒子。在室温下提取。采用紫外可见吸收光谱、X射线衍射、透射电镜和傅里叶变换红外光谱等方法研究了提取物对银纳米粒子形成的影响。紫外和可见吸收光谱的结果表明，在430 nm处的银纳米粒子（AgNP）的表面为中心的强共振。利用傅里叶变换红外光谱法对杭白菊进行光谱研究。萃取物在合成过程中充当还原剂和稳定剂。X射线衍射分析证实所合成的AgNP为单晶，与透射电子显微镜的结果相一致。在透射电子显微镜图像中还观察到具有约20 nm-50 nm尺寸的水溶性AgNP。采用琼脂稀释法和抑菌试验研究了所合成的AgNP的杀菌性能。结果表明，AgNP对金黄色葡萄球菌和大肠杆菌具有较强的杀菌活性，对革兰氏阴性菌具有较强的抑菌活性，对革兰氏阳性菌的抑菌活性呈剂量依赖性，为临床应用提供了一种具有杀菌性能的预防交叉感染的超声凝胶剂。
Eco-friendly green synthesis with plant extracts plays a very important role in nanotechnology, without any harmful chemicals. In this report, the synthesis of water-soluble silver nanoparticles was developed by treating silver ions with Chrysanthemum morifolium Ramat. extract at room temperature. The effect of the extract on the formation of silver nanoparticles was characterized by ultraviolet and visible absorption spectroscopy, X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. The ultraviolet and visible absorption spectroscopy results show a strong resonance centered on the surface of silver nanoparticles (AgNP) at 430 nm. The Fourier transform infrared spectroscopy spectral study demonstrates Chrysanthemum morifolium Ramat. extract acted as the reducing and stabilizing agent during the synthesis. The X-ray diffraction analysis confirmed that the synthesized AgNP are single crystallines, corresponding with the result of transmission electron microscopy. Water-soluble AgNP, with an approximate size of 20 nm–50 nm were also observed in the transmission electron microscopy image. The bactericidal properties of the synthesized AgNP were investigated using the agar-dilution method and the growth-inhibition test. The results show the AgNP had potent bactericidal activity on Staphylococcus aureus and Escherichia coli, as well as a strong antibacterial activity against gram-negative bacteria, as compared to gram-positive bacteria with a dose-dependent effect, thus providing a clinical ultrasound gel with bactericidal property for prevention of cross infections.