Phylogenomic analyses reveal the diversity of laccase-coding genes in Fonsecaea genomes.
Phylogenomic analyses reveal the diversity of laccase-coding genes in Fonsecaea genomes.
复制标题
系统基因组分析揭示了丰塞卡菌基因组中漆酶编码基因的多样性。
DOI：
10.1371/journal.pone.0171291
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
de Hoog S
中科院分区：
文献类型：
--
作者：
Moreno LF;Feng P;Weiss VA;Vicente VA;Stielow JB;de Hoog S
丰塞卡菌属包含具有临床意义的黑酵母样真菌，包括着色芽生菌病和脑褐丝菌病的病原体。黑色素的存在以及单芳烃和烷基苯的同化已被认为是毒力因子。多铜氧化酶（MCO）是一个酶家族，包括漆酶、亚铁氧化酶和抗坏血酸氧化酶，能够催化各种芳香族有机化合物的氧化，将分子氧还原成水。此外，漆酶是真菌黑色素的产生所必需的，真菌黑色素是一种细胞壁黑色素，被认为是黑酵母样真菌致病性和极耐受性的关键聚合物。尽管此前已在许多木材腐烂真菌中报道了漆酶的活性，但丰塞卡菌中漆酶基因的多样性尚未得到评估。在这项研究中，我们鉴定并表征了漆酶编码基因，并确定了它们在五种临床和环境 Fonsecaea 物种中的基因组位置。严格意义上漆酶的鉴定将为 Fonsecaea 中的碳获取策略和黑色素产生提供见解。
The genus Fonsecaea comprises black yeast-like fungi of clinical relevance, including etiologic agents of chromoblastomycosis and cerebral phaeohyphomycosis. Presence of melanin and assimilation of monoaromatic hydrocarbons and alkylbenzenes have been proposed as virulence factors. Multicopper oxidase (MCO) is a family of enzymes including laccases, ferroxidases and ascorbate oxidases which are able to catalyze the oxidation of various aromatic organic compounds with the reduction of molecular oxygen to water. Additionally, laccases are required for the production of fungal melanins, a cell-wall black pigment recognized as a key polymer for pathogenicity and extremotolerance in black yeast-like fungi. Although the activity of laccase enzymes has previously been reported in many wood-rotting fungi, the diversity of laccase genes in Fonsecaea has not yet been assessed. In this study, we identified and characterized laccase-coding genes and determined their genomic location in five clinical and environmental Fonsecaea species. The identification of laccases sensu stricto will provide insights into carbon acquisition strategies as well as melanin production in Fonsecaea.