Cellular retinol-binding protein (type two) is abundant in human small intestine.
Cellular retinol-binding protein (type two) is abundant in human small intestine.
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DOI：
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发表时间：
1987-06
影响因子：
6.5
通讯作者：
D. Ong;D. Page
中科院分区：
文献类型：
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作者：
D. Ong;D. Page
研究发现，人类小肠中含有一种视黄醇结合蛋白，类似于在大鼠中描述的肠道特异性细胞视黄醇结合蛋白2型[CRBP (II)]。这种新检测到的人蛋白在免疫化学上与先前描述的人细胞视黄醇结合蛋白不同，但在免疫化学上与大鼠CRBP （II）相似。部分纯化的蛋白结合视黄醇，其荧光激发和发射光谱与与纯人CRBP结合的视黄醇的光谱不同，但与与大鼠CRBP结合的视黄醇的光谱相似（II）。利用抗大鼠CRBP （II）血清，免疫组化方法可将人CRBP （II）定位于绒毛相关肠细胞。该蛋白含量丰富，占空肠黏膜提取物中可溶性蛋白总量的0.4%。该蛋白可能在维生素A的吸收和必要的肠道代谢中起重要作用。
Human small intestine was found to contain a retinol-binding protein similar to the gut-specific cellular retinol-binding protein, type two [CRBP (II)], described in the rat. This newly detected human protein was immunochemically distinct from human cellular retinol binding protein previously described but immunochemically similar to rat CRBP (II). The partially purified protein bound retinol and exhibited fluorescence excitation and emission spectra distinct from those spectra for retinol bound to pure human CRBP but similar to the spectra for retinol bound to rat CRBP (II). Human CRBP (II) could be localized to the villus-associated enterocytes by immunohistochemistry, using antiserum against rat CRBP (II). The protein was abundant representing 0.4% of the total soluble protein in a jejunum mucosal extract. This protein may play an important role in the absorption and necessary intestinal metabolism of vitamin A.