Identification of culturable bacterial endophyte community isolated from tissues of Vitis vinifera "Glera"
Identification of culturable bacterial endophyte community isolated from tissues of Vitis vinifera "Glera"
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DOI：
10.1080/11263504.2014.916364
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发表时间：
2014-01-01
期刊：
影响因子：
2
通讯作者：
Baldan, B.
中科院分区：
文献类型：
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作者：
Baldan, E.;Nigris, S.;Baldan, B.
内生菌是一种微生物，它能在植物的内部组织中定植，不会引起负面影响，并能为植物健康和产量提供益处。本研究从表面灭菌的植物组织中分离出了定植于葡萄藤营养器官中的可培养内生细菌群落，并对其进行了分子生物学鉴定。来自科内利亚诺-瓦尔多比亚德内DOCG地区六个不同葡萄园的葡萄属葡萄“格拉”的根、芽和叶（韦内托，意大利），成功分离出381株可培养菌株;扩增核糖体DNA限制性分析和核苷酸测序显示，约30%的内生菌群落属于芽孢杆菌属，最具代表性;其它属如葡萄球菌属、微杆菌属、类芽孢杆菌属、短杆菌属、寡养单胞菌属、多变菌属、微球菌属和土壤球菌属被鉴定。内生菌群落组成在每一个葡萄是不同的其他内生菌种群生活在葡萄藤植物来自不同的葡萄园，此外，细菌组成的变化取决于采样的季节。上述数据突出了居住在Glera葡萄藤上的可培养细菌物种的巨大多样性，并为菌株的表征和选择开辟了道路，这些菌株可能用于改善葡萄园的植物生长和产量管理，植物对胁迫的反应，生物控制和生物施肥。
Endophytes are micro-organisms that colonize the internal tissues of plants without inducing signs of negative effects and that can provide benefits to plant health and yield. In the present work, the culturable bacterial endophyte community, colonizing vegetative organs of grapevine, was isolated from surface-sterilized plant tissues and characterized by molecular methods. From roots, shoots and leaves of Vitis vinifera "Glera", located in six different vineyards throughout the Conegliano-Valdobbiadene DOCG area (Veneto, Italy), 381 culturable strains were successfully isolated; amplified ribosomal DNA restriction analysis and nucleotide sequencing showed that approximately 30% of the endophyte community belonged to the genus Bacillus, which was the most represented; other genera such as Staphylococcus, Microbacterium, Paenibacillus, Curtobacterium, Stenotrophomonas, Variovorax, Micrococcus and Agrococcus were identified. Endophyte community composition within each vine was different in respect to other endophyte populations living in grapevine plants coming from different vineyards; moreover, the bacterial composition changed depending on the season of sampling. The above data highlight the great diversity of culturable bacterial species inhabiting Glera grapevines and open the way for a characterization and selection of strains that could potentially be used to improve the vineyard management for plant growth and yield, plant responses to stresses, biocontrol and biofertilization.