The effect of G-CSF in a myocardial ischemia reperfusion model rat.
The effect of G-CSF in a myocardial ischemia reperfusion model rat.
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DOI：
10.2152/jmi.54.177
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发表时间：
2007-02-01
期刊：
影响因子：
--
通讯作者：
Ito, Susumu
中科院分区：
文献类型：
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作者：
Ieishi, Kiyoshi;Nomura, Masahiro;Ito, Susumu
目得：据报道，G-CSF给药通过减少心肌梗塞模型大鼠的梗塞面积来改善心脏功能。本研究通过结扎大鼠左冠状动脉前支制备心肌梗死模型。方法：结扎12周龄Wistar大鼠冠状动脉左前降支建立心肌梗死模型，观察G-CSF对缺血再灌注心肌细胞损伤的治疗作用。对完全结扎的大鼠（MI-G组，n=6）和结扎后30分钟再灌注的大鼠（R-G组，n = 6），每天以100 μ g/kg/天的剂量皮下给予G-CSF，持续5天。将生理盐水皮下给予具有完全结扎和再灌注的大鼠（分别为MI-C和R-C组，各自n=6），作为对照。4周后，心肌梗死面积比（%），心脏功能超声心动图（左心室射血分数），心肌组织病理学诊断进行了比较，各组之间的结果：MI-G和MI-C组之间的心肌梗死病变，心肌壁厚度，或左心室射血分数的残留心肌的比例没有显着差异。相反，R-G组的梗死面积、心肌壁厚度和左室射血分数较R-C组显著改善（p
PURPOSE: It has been reported that G-CSF administration improves cardiac function by reducing the area of the infarct in a myocardial infarction model rat. In the present study, myocardial infarction model rats, produced by ligation of the left anterior coronary artery, were prepared. The G-CSF effect for treating cardiac muscle cell disorders by ischemia reperfusion was studied.METHODS: Myocardial infarction model rats were produced by ligation of the left anterior descending coronary artery in 12-week-old Wistar rats. G-CSF was administered subcutaneously daily at a dose of 100 microg/kg/day for 5 days to rats with a complete ligation (MI-G group, n=6) and rats in which the ligated coronary artery was reperfused 30 minutes after the ligation (R-G group, n=6). Physiological saline was subcutaneously administered to rats with a complete ligation and reperfusion (MI-C and R-C groups, respectively, n=6 each), as controls. After 4 weeks, the infarct area ratio (%), cardiac function on echocardiography (left ventricular ejection fraction), and a myocardial histopathological diagnosis were carried out and the results compared among the groups.RESULTS: No significant differences were found in the proportion of the residual heart muscle in the infarct lesion, myocardial wall thickness, or left ventricular ejection fraction between the MI-G and MI-C groups. In contrast, the infarct area, myocardial wall thickness, and left ventricular ejection fraction were significantly improved in the R-G group compared to the R-C group (p