miR-532-3p promotes hepatocellular carcinoma progression by targeting PTPRT
miR-532-3p promotes hepatocellular carcinoma progression by targeting PTPRT
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miR-532-3p通过靶向PTPRT促进肝细胞癌进展
DOI：
10.1016/j.biopha.2018.10.145
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发表时间：
2019-01-01
影响因子：
7.5
通讯作者：
Han, Shaoshan
中科院分区：
文献类型：
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作者：
Wang, Yufeng;Yang, Zhencun;Han, Shaoshan
背景：miR-532-3p 的异常表达参与多种癌症的进展和发展，而 miR-532-3p 在肝细胞癌（HCC）中尚未有报道。本研究的目的是阐明miR-532-3p在HCC进展中的功能。方法：通过HCC组织和细胞系中的实时PCR和数据库分析来检测miR-532-3p在HCC中的表达。然后，统计测量miR-532-3p与HCC患者临床病理特征和预后的关系。随后，我们尝试通过伤口愈合实验、Transwell实验、MTT实验和EdU实验观察miR-532-3p对HCC细胞迁移、侵袭和增殖的影响。此外，还通过生物信息学工具、数据库分析、荧光素酶报告基因检测和拯救实验来探索HCC中miR-532-3p的靶点，并探讨该靶点是否介导miR-532-3p对HCC细胞的影响。结果：我们的研究结果和数据库数据一致表明，HCC中miR-532-3p的表达水平较高。此外，发现高miR-532-3p表达与较大的肿瘤大小（P = 0.0027）、血管侵犯的存在（P = 0.015）和晚期TNM分期（P = 0.015）密切相关。此外，体外实验表明miR-532-3p促进HCC细胞的迁移、侵袭和增殖。此外，受体蛋白酪氨酸磷酸酶 T (PTPRT) 被确定为 HCC 细胞中 miR-532-3p 的靶标和介质。结论：我们的结果表明，在 HCC 中频繁上调的 miR-532-3p 通过靶向 PTPRT 有助于 HCC 细胞的迁移和增殖。
Background: Aberrant expression of miR-532-3p was involved in progression and development of multiple cancers, whereas miR-532-3p has not been reported in hepatocellular carcinoma (HCC). The aim of this study was to elucidate the functions of miR-532-3p in progression of HCC.Methods: Real-time PCR in HCC tissues and cell lines and database analysis were conducted for detection of the expression of miR-532-3p in HCC. Then, the association of miR-532-3p with clinicopathological features and prognosis of HCC patients were statistically measured. Subsequently, we attempted to observe the effects of miR-532-3p on migration, invasion and proliferation of HCC cells by Wound healing assay, Transwell assays, MTT assay and EdU assay. Furthermore, bioinformatics tools, database analysis, luciferase reporter gene assay and rescue experiments were conducted to explore the target of miR-532-3p in HCC, and to explore whether the target mediated the effects of miR-532-3p on HCC cells.Results: Our findings and data from databases consistently indicated that the miR-532-3p expression level was higher in HCC. In addition, high miR-532-3p expression was found to be closely related to larger tumor size (P = 0.0027), presence of vascular invasion (P = 0.015), and advanced TNM stage (P = 0.015). In addition, experiments in vitro revealed that miR-532-3p promotes migration, invasion and proliferation of HCC cells. Furthermore, receptor protein tyrosine phosphatase T (PTPRT) was identified as the target and mediator of miR-532-3p in HCC cells.Conclusion: Our results demonstrate that miR-532-3p, which is frequently up-regulated in HCC, contributes to HCC cells mobility and proliferation through targeting PTPRT.