Immunoreactive corticotropin and corticotropin-releasing factor in human hypothalamus, adrenal, lung cancer, and pheochromocytoma.
Immunoreactive corticotropin and corticotropin-releasing factor in human hypothalamus, adrenal, lung cancer, and pheochromocytoma.
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人下丘脑、肾上腺、肺癌和嗜铬细胞瘤中的免疫反应性促肾上腺皮质激素和促肾上腺皮质激素释放因子。
DOI：
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发表时间：
1984
影响因子：
5.8
通讯作者：
N. Sasano
中科院分区：
文献类型：
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作者：
T. Suda;N. Tomori;F. Tozawa;H. Demura;K. Shizume;T. Mouri;Y. Miura;N. Sasano
用放射免疫法、免疫亲和层析法和凝胶过滤层析法对人下丘脑、肾上腺(皮质和髓质)、肺癌和嗜铬细胞瘤的促肾上腺皮质激素释放因子(I-CRF)和促肾上腺皮质激素释放激素(I-ACTH)进行了检测。这些组织中存在I-CRF和I-ACTH。I-ACTH在肾上腺、嗜铬细胞瘤和肺癌中的凝胶过滤显示，与下丘脑相反，存在更多的大分子形式的I-ACTH。I-CRF在这些组织中的凝胶过滤显示，主峰洗脱在合成的大鼠CRF的位置。该主峰的高效液相色谱显示，在合成的大鼠CRF和氧化型CRF的位置有两种洗脱成分。这些洗脱位置在所有组织中都是相同的，并且与下丘脑中的位置相同。这些结果表明，这些组织中存在I-ACTH和I-CRF，脑外CRF与下丘脑CRF相同。
Immunoreactive corticotropin-releasing factor (I-CRF) and ACTH (I-ACTH) were examined using RIA, immunoaffinity chromatography, and gel filtration chromatography in human hypothalamus, adrenal (cortex and medulla), lung cancer, and pheochromocytoma. I-CRF and I-ACTH were present in these tissues. Gel filtration of I-ACTH in the adrenal, pheochromocytoma, and lung cancer showed the presence of larger amounts of I-ACTH with large molecular weight forms in contrast to the hypothalamus. Gel filtration of I-CRF in these tissues showed the main peak eluted at the position of synthetic rat CRF. High performance liquid chromatography of this main peak showed two components which eluted in the positions of synthetic rat CRF and oxidized CRF. These elution positions were the same in all tissues and identical with those in the hypothalamus. These results suggest the presence of I-ACTH and I-CRF in these tissues and that CRF outside the brain is identical to hypothalamic CRF.