Prevalence of the ST239 clone of methicillin-resistant Staphylococcus aureus and differences in antimicrobial susceptibilities of ST239 and ST5 clones identified in a Korean hospital
Prevalence of the ST239 clone of methicillin-resistant Staphylococcus aureus and differences in antimicrobial susceptibilities of ST239 and ST5 clones identified in a Korean hospital
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DOI：
10.1128/jcm.43.8.3610-3614.2005
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发表时间：
2005-08-01
影响因子：
9.4
通讯作者：
Lee, JC
中科院分区：
文献类型：
--
作者：
Cha, HY;Moon, DC;Lee, JC
2001年至2004年在韩国大邱的一所大学医院获得的188株非重复耐甲氧西林金黄色葡萄球菌（MRSA）分离株，通过分子分型技术（包括多位点序列分型、spaA分型、葡萄球菌染色体盒mec（SCCmec）分型和脉冲场凝胶电泳（PFGE））分析其克隆型。检查了它们的抗微生物能力。根据序列分型，大多数（87%）MRSA分离株属于序列类型239（ST 239; n = 100; 53%）和ST 5（n = 63，34%）。属于ST 239的MRSA分离株在基因型上是同质的，而属于ST 5的MRSA分离株在spaA型、SCCmec型和PFGE模式上显示出变异。ST 239菌株对甲氧苄啶、磺胺甲恶唑、妥布霉素、庆大霉素、红霉素和四环素的耐药率明显高于ST 5菌株（P < 0.05）。这项研究表明，ST 239克隆，而很少在韩国检测到，是普遍的，ST 239克隆的抗菌药物敏感性是显着不同的ST 5克隆。
A total of 188 nonduplicate methicillin-resistant Staphylococcus aureus (MRSA) isolates obtained between 2001 and 2004 in a university hospital in Daegu, Korea, were analyzed for their clonal types by molecular typing techniques, including multilocus sequence typing, spaA typing, staphylococcal chromosomal cassette mec (SCCmec) typing, and pulsed-field gel electrophoresis (PFGE). They were examined for their antimicrobial susceptibilities. The majority (87%) of MRSA isolates belonged to sequence type 239 (ST239; n = 100; 53%) and ST5 (n = 63, 34%) on the basis of sequence typing. MRSA isolates belonging to ST239 were genotypically homogeneous, while those belonging to ST5 showed variations in spaA type, SCCmec type, and PFGE patterns. The rates of resistance of the MRSA isolates belonging to ST239 to trimethoprim, sulfamethoxazole, tobramycin, gentamicin, erythromycin, and tetracycline were significantly higher than those of the isolates belonging to ST5 (P < 0.05). This study demonstrated that the ST239 clone, while rarely detected in Korea, was prevalent and that the antimicrobial susceptibility of the ST239 clone was significantly different from that of the ST5 clone.