Endophytic Diaporthe associated with Citrus: A phylogenetic reassessment with seven new species from China
Endophytic Diaporthe associated with Citrus: A phylogenetic reassessment with seven new species from China
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DOI：
10.1016/j.funbio.2015.02.006
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发表时间：
2015-05-01
期刊：
影响因子：
2.5
通讯作者：
Li, Hongye
中科院分区：
文献类型：
--
作者：
Huang, Feng;Udayanga, Dhanushka;Li, Hongye
柑桔根枯病菌与柑桔的黑变病、茎端腐烂病和流胶病等病害密切相关。然而，很少有人知道的发生种间充质作为内生菌和saprobes。本研究从我国栽培柑橘中分离到58个间壁孢菌菌株，其中内生菌株44个。分析了核核糖体内转录间隔区（ITS）、翻译延伸因子1-α（EF 1-α）、β-微管蛋白（TUB）和组蛋白-3（HIS）基因区域的部分序列，以确定收集的分离株的种属。在4个基因区域的联合分析中，这些菌株在间沟菌中可分为16个不同的分支。根据分子遗传学和形态学鉴定，分离物分别属于槟榔间座壳种复合体、柑橘间座壳、柑橘夏间座壳、柑橘黄间座壳、内生间座壳、黑间座壳、香港间座壳和大豆间座壳。描述了柑桔属7个新种，即双锥散囊壳Diaporthe biconispora、双点散囊壳Diaporthe biguttulata、盘状散囊壳Diaporthe discoidispora、多点散囊壳Diaporthe multigutullata、卵圆散囊壳Diaporthe ovalispora、近棒散囊壳Diaporthe subclavata和温州散囊壳Diaporthe unshiuensis，并附有描述和插图。(C)2015由Elsevier Ltd代表英国真菌学会出版。
Phytopathogenic species of Diaporthe are associated with melanose, stem-end rot and gummosis diseases of Citrus. However, little is known about the occurrence of species of Diaporthe as endophytes and saprobes. In this study, we obtained 58 strains of Diaporthe, including 44 endophytic isolates from cultivated Citrus in China. The nuclear ribosomal internal transcribed spacer (ITS), partial sequences of translation elongation factor 1-alpha (EF1-alpha), beta-tubulin (TUB), and histone-3 (HIS) gene regions were analysed to determine the species of the isolates collected. In combined analysis of four gene regions, these strains grouped in 16 distinct clades in Diaporthe. The isolates were identified in Diaporthe arecae species complex, Diaporthe citri, Diaporthe citriasiana, Diaporthe citrichinensis, Diaporthe endophytica, Diaporthe eres, Diaporthe hongkongensis, and Diaporthe sojae based on molecular phylogeny and morphology. Seven new species are described from Citrus namely, Diaporthe biconispora, Diaporthe biguttulata, Diaporthe discoidispora, Diaporthe multigutullata, Diaporthe ovalispora, Diaporthe subclavata, and Diaporthe unshiuensis with descriptions and illustrations. (C) 2015 Published by Elsevier Ltd on behalf of The British Mycological Society.