Resveratrol Interaction with Lipid Bilayers: A Synchrotron X-ray Scattering Study
Resveratrol Interaction with Lipid Bilayers: A Synchrotron X-ray Scattering Study
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DOI：
10.1021/acs.langmuir.6b03591
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发表时间：
2016-12-06
期刊：
影响因子：
3.9
通讯作者：
Reis, Salette
中科院分区：
文献类型：
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作者：
Neves, Ana Rute;Nunes, Claudia;Reis, Salette
白藜芦醇属于一大类具有生物活性的多酚化合物，具有多种有益健康的作用，包括抗氧化活性、抗炎作用、心血管保护、神经保护和癌症化学预防。在本研究中，评估了上述白藜芦醇的作用是由白藜芦醇膜相互作用和脂质双层结构修饰引起的可能性。在这种情况下，白藜芦醇可能选择性地与生物膜中的脂质结构域相互作用，从而调节锚定蛋白的定位并控制其细胞内级联反应。本研究在同步加速器中进行，利用小角和广角x射线散射（SAXS和WAXS）技术研究了白藜芦醇对双层脂质结构域结构组织的影响。采用不同摩尔比的鸡蛋l - α -磷脂酰胆碱（EPC）、胆固醇（CHOL）和鞘磷脂（SM）组成的模拟膜体系，研究了白藜芦醇对膜的有序和结构的影响。结果表明，白藜芦醇诱导相分离，促进EPC、EPC:CHOL[4:1]和EPC:CHOL:SM[1:1:1]双分子层中脂质结构域的形成，使膜具有一定的结构组织。因此，白藜芦醇通过诱导脂筏的组织来控制双分子层的脂质堆积。此外，脂质结构域的形成对于调节许多受体、跨膜蛋白和酶的活性非常重要，这些活性取决于膜的结构组织和这些组织结构域的存在与否。因此，这一证据可以解释白藜芦醇的治疗效果。
Resveratrol belongs to the large group of biologically active polyphenol compounds, with several beneficial health effects including antioxidant activity, anti-inflammatory action, cardiovascular protection, neuroprotection, and cancer chemoprevention. In the present study, the possibility that the effects of resveratrol described above are caused by resveratrol membrane interactions and structural modifications of lipid bilayers is evaluated. In this context, it is possible that resveratrol interacts selectively with lipid domains present in biological membranes, thereby modulating the localization of the anchored proteins and controlling their intracellular cascades. This study was conducted in a synchrotron particle accelerator, where the influence of resveratrol in the structural organization of lipid domains in bilayers was investigated using small- and wide-angle X-ray scattering (SAXS and WAXS) techniques. Membrane mimetic systems composed of egg L-alpha-phosphatidylcholine (EPC), cholesterol (CHOL), and sphingomyelin (SM), with different molar ratios, were used to access the effects of resveratrol on the order and structure of the membrane. The results revealed that resveratrol induces phase separation, promoting the formation of lipid domains in EPC, EPC:CHOL [4:1], and EPC:CHOL:SM [1:1:1] bilayers, which brings some structural organization to membranes. Therefore, resveratrol controls lipid packing of bilayers by inducing the organization of lipid rafts. Moreover, the formation of lipid domains is important for modulating the activity of many receptors, transmembrane proteins, and enzymes whose activity depends on the structural organization of the membrane and on the presence or absence of these organized domains. This evidence can thereby explain the therapeutic effects of resveratrol.