Changes in estrogen receptor expression in the chick thymus during late embryonic development
Changes in estrogen receptor expression in the chick thymus during late embryonic development
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胚胎发育后期鸡胸腺雌激素受体表达的变化
DOI：
10.1111/asj.12114
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发表时间：
2014
影响因子：
2
通讯作者：
Yasuhiro Kondo
中科院分区：
文献类型：
--
作者：
Masafumi Katayama;Tomokazu Fukuda;Toshimitsu Hatabu;Kiyoaki Narabara;Asaki Abe;Yasuhiro Kondo
在鸡中，虽然雌激素受体(ER)被报道与免疫过程有关，但关于ER在与淋巴细胞发育相关的组织中表达差异的详细信息尚不完全清楚，特别是在发育阶段。为了进一步了解这种免疫学关系，我们用半定量聚合酶链式反应方法检测了发育阶段雏鸡胸腺组织中ER的表达水平。免疫组织化学方法检测ER表达细胞。结果表明，胚胎16d时ER的表达水平升高，20d时表达水平下降，且16d时公鸡的ER值显著高于母鸡。免疫反应细胞的发生率也出现了16d的升高和20d的下降的现象，但细胞的升高出现的时间延迟了1d。本研究揭示了雏鸡胸腺发育过程中ER表达和ER阳性细胞发生率的变化。
In chickens, although estrogen receptors (ER) are reported to be associated with the immunological processes, detailed information about the differences in ER expression in the tissues related to the development of lymphocytes is not fully known, especially during the developmental stage. To learn more about this immunological relationship, we used semi‐quantitative polymerase chain reaction method to detect theERexpression levels in the thymus tissues of chicks during the developmental stage. Furthermore, ER‐expressing cells were detected by immunohistochemistry. The results of this study show that the expression level ofERincreased on embryonic day 16 and decreased on day 20. Furthermore,ERexpression was significantly higher in male than in female chickens at day 16. The increased expression on day 16 and decreased level on day 20 were also reproduced in the incidence of immunoreactive cells, although there was a 1‐day delay in the elevated incidence of the cells. This study revealed the changes in ER expression and the incidence of ER‐positive cells in the thymus of chickens during the developmental stage.