Polymerase chain reaction assay targeting cytochrome b gene for the detection of dog meat adulteration in meatball formulation
Polymerase chain reaction assay targeting cytochrome b gene for the detection of dog meat adulteration in meatball formulation
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DOI：
10.1016/j.meatsci.2014.03.011
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发表时间：
2014-08-01
期刊：
影响因子：
7.1
通讯作者：
Hanapi, Ummi Kalthum
中科院分区：
文献类型：
--
作者：
Rahman, Md Mahfujur;Ali, Md Eaqub;Hanapi, Ummi Kalthum
开发了一种用于评估肉丸中狗肉掺假的聚合酶链反应（PCR）测定法。该检测从纯背景、原始背景、加工背景和混合背景中选择性扩增了犬线粒体细胞色素 b 基因的 100 bp 区域。该测定的特异性针对 11 种动物和 3 种植物进行了测试，这些植物通常可用于肉丸配方。该测定的稳定性在可分解目标 DNA 的广泛高压灭菌条件下得到了证实。对即食鸡肉和牛肉丸进行的盲测表明，该方法可以使用从差别处理的肉丸中提取的 0.04 ng 狗 DNA，在复杂基质下重复检测 0.2% 的犬肉组织。该测定的简单性、稳定性和敏感性表明它可以用于清真食品工业，用于加工食品中犬衍生物的鉴定。 (C) 2014 Elsevier Ltd. 保留所有权利。
A polymerase chain reaction (PCR) assay for the assessment of dog meat adulteration in meatballs was developed. The assay selectively amplified a 100-bp region of canine mitochondrial cytochrome b gene from pure, raw, processed and mixed backgrounds. The specificity of the assay was tested against 11 animals and 3 plants species, commonly available for meatball formulation. The stability of the assay was proven under extensively autoclaving conditions that breakdown target DNA. A blind test from ready to eat chicken and beef meatballs showed that the assay can repeatedly detect 0.2% canine meat tissues under complex matrices using 0.04 ng of dog DNA extracted from differentially treated meatballs. The simplicity, stability and sensitivity of the assay suggested that it could be used in halal food industry for the authentication of canine derivatives in processed foods. (C) 2014 Elsevier Ltd. All rights reserved.