Raman chemical imaging, a new tool in kidney stone structure analysis: Case-study and comparison to Fourier Transform Infrared spectroscopy.
Raman chemical imaging, a new tool in kidney stone structure analysis: Case-study and comparison to Fourier Transform Infrared spectroscopy.
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DOI：
10.1371/journal.pone.0201460
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Ziemons E
中科院分区：
文献类型：
--
作者：
Castiglione V;Sacré PY;Cavalier E;Hubert P;Gadisseur R;Ziemons E
除了结石成分之外，肾结石的结构还可以提供临床信息。拉曼化学成像是一种用于生成样品中成分分布的二维图的技术。我们的目的是确定拉曼化学成像在尿路结石分析中的应用。使用共焦拉曼显微分光光度计通过拉曼化学成像分析了十四个结石。它们是根据其异质组成和形态进行选择的。对整个结石部分进行拉曼化学成像。获取数据后，通过 MCR-ALS 进行基线校正和分析。然后将结果与傅里叶变换红外光谱获得的光谱进行比较。拉曼化学成像成功地鉴定了每个样品的几乎所有化学成分，包括一水和二水草酸钙、无水和二水尿酸、磷灰石、鸟粪石、透钙磷石以及稀有化学物质如辉磷矿、尿酸铵和药物。然而，由于巨大的自发荧光背景和这些较差的拉曼散射体的浓度较小，无法检测到蛋白质。即使碳磷灰石和草酸钙占总结石的比例不到 5%，也能正确检测到。此外，拉曼化学成像提供了结石内成分的分布：准确识别了细胞核以及其他成分的薄层。在一个样品的中心正确观察到二水草酸钙向一水草酸钙的转化。一水草酸钙根据其定位具有不同的拉曼光谱。与红外光谱相比，拉曼化学成像在识别肾结石成分方面显示出良好的准确性。该分析对于确定宝石内成分的组织也很有用，这有助于定位少量成分，例如细胞核。然而，这种分析非常耗时，因此更适合研究而不是常规分析。
The kidney stone’s structure might provide clinical information in addition to the stone composition. The Raman chemical imaging is a technology used for the production of two-dimension maps of the constituents' distribution in samples. We aimed at determining the use of Raman chemical imaging in urinary stone analysis. Fourteen calculi were analyzed by Raman chemical imaging using a confocal Raman microspectrophotometer. They were selected according to their heterogeneous composition and morphology. Raman chemical imaging was performed on the whole section of stones. Once acquired, the data were baseline corrected and analyzed by MCR-ALS. Results were then compared to the spectra obtained by Fourier Transform Infrared spectroscopy. Raman chemical imaging succeeded in identifying almost all the chemical components of each sample, including monohydrate and dihydrate calcium oxalate, anhydrous and dihydrate uric acid, apatite, struvite, brushite, and rare chemicals like whitlockite, ammonium urate and drugs. However, proteins couldn't be detected because of the huge autofluorescence background and the small concentration of these poor Raman scatterers. Carbapatite and calcium oxalate were correctly detected even when they represented less than 5 percent of the whole stones. Moreover, Raman chemical imaging provided the distribution of components within the stones: nuclei were accurately identified, as well as thin layers of other components. Conversion of dihydrate to monohydrate calcium oxalate was correctly observed in the centre of one sample. The calcium oxalate monohydrate had different Raman spectra according to its localization. Raman chemical imaging showed a good accuracy in comparison with infrared spectroscopy in identifying components of kidney stones. This analysis was also useful in determining the organization of components within stones, which help locating constituents in low quantity, such as nuclei. However, this analysis is time-consuming, making it more suitable for research studies rather than routine analysis.
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影响因子：
3.1
作者：
Englert, Kate M.;McAteer, James A.;Williams, James C., Jr.
通讯作者：
Williams, James C., Jr.
DOI：
10.1136/bmj.e5287
发表时间：
2012-08-29
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Alexander RT;Hemmelgarn BR;Wiebe N;Bello A;Morgan C;Samuel S;Klarenbach SW;Curhan GC;Tonelli M;Alberta Kidney Disease Network
通讯作者：
Alberta Kidney Disease Network
影响因子：
--
作者：
Daudon, Michel;Jungers, Paul
通讯作者：
Jungers, Paul
影响因子：
--
作者：
Daudon, Michel;Bouzidi, Hassan;Bazin, Dominique
通讯作者：
Bazin, Dominique
影响因子：
14.8
作者：
Felten, Judith;Hall, Hardy;Gorzsas, Andras
通讯作者：
Gorzsas, Andras