Uropathogenic Escherichia coli (UPEC) strains may carry virulence properties of diarrhoeagenic E-coli
Uropathogenic Escherichia coli (UPEC) strains may carry virulence properties of diarrhoeagenic E-coli
复制标题
DOI：
10.1111/j.1574-695x.2008.00388.x
复制
发表时间：
2008-04-01
影响因子：
--
通讯作者：
Gomes, Tania A. T.
中科院分区：
文献类型：
--
作者：
Abe, Cecilia M.;Salvador, Fabia A.;Gomes, Tania A. T.
目的分析引起尿路感染的大肠埃希菌是否与致病性大肠埃希菌具有相同的毒力特性。大肠杆菌（DEC）致病型和识别其遗传多样性，检测了225株UPEC菌株的DEC和UPEC的各种特性（与HeLa细胞的相互作用类型、血清型和30个毒力基因的存在）的存在。粘附模式和血清型之间没有相关性。共发现45个血清群，但64%的菌株属于12个血清群（O1、O2、O4、O6、O7、O14、O15、O18、O21、O25、O75和O175）之一，并携带UPEC毒力基因（pap、hly、aer、sfa、cnf）。发现的DEC基因是：aap、aatA、aggC、agg3C、aggR、astA、eae、ehly、iha、irp2、lpfA（O113）、pet、pic、pilS和shf。16株菌株具有肠聚集性E.大肠杆菌（EAEC），DEC致病型之一。总之，某些UPEC菌株可能携带DEC毒力特性，主要与EAEC致病型相关。这一发现提出了至少一些粪便EAEC菌株可能代表潜在的尿路病原体的可能性。或者，某些UPEC菌株可能已获得EAEC特性，成为腹泻的潜在原因。
To analyze whether Escherichia coli strains that cause urinary tract infections (UPEC) share virulence characteristics with the diarrheagenic E. coli (DEC) pathotypes and to recognize their genetic diversity, 225 UPEC strains were examined for the presence of various properties of DEC and UPEC (type of interaction with HeLa cells, serogroups and presence of 30 virulence genes). No correlation between adherence patterns and serogroups was observed. Forty-five serogroups were found, but 64% of the strains belonged to one of the 12 serogroups (O1, O2, O4, O6, O7, O14, O15, O18, O21, O25, O75, and O175) and carried UPEC virulence genes (pap, hly, aer, sfa, cnf). The DEC genes found were: aap, aatA, aggC, agg3C, aggR, astA, eae, ehly, iha, irp2, lpfA(O113), pet, pic, pilS, and shf. Sixteen strains presented aggregative adherence and/or the aatA sequence, which are characteristics of enteroaggregative E. coli (EAEC), one of the DEC pathotypes. In summary, certain UPEC strains may carry DEC virulence properties, mostly associated to the EAEC pathotype. This finding raises the possibility that at least some faecal EAEC strains might represent potential uropathogens. Alternatively, certain UPEC strains may have acquired EAEC properties, becoming a potential cause of diarrhoea.