Defensive Armor of Potato Tubers: Nonpolar Metabolite Profiling, Antioxidant Assessment, and Solid-State NMR Compositional Analysis of Suberin-Enriched Wound-Healing Tissues.
Defensive Armor of Potato Tubers: Nonpolar Metabolite Profiling, Antioxidant Assessment, and Solid-State NMR Compositional Analysis of Suberin-Enriched Wound-Healing Tissues.
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DOI：
10.1021/acs.jafc.5b03206
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发表时间：
2015-08-05
影响因子：
6.1
通讯作者：
Stark RE
中科院分区：
文献类型：
--
作者：
Dastmalchi K;Kallash L;Wang I;Phan VC;Huang W;Serra O;Stark RE
马铃薯块茎的种植、储存和分配受到机械损伤和次优愈合的影响。调查伤口愈合的进展与对比russeting模式的品种，极性组织提取物的代谢产物谱先前报道的补充GC/MS测量非极性提取物和定量13 C NMR的界面固体悬浮液。区分品种类型和伤口愈合时间点的潜在标记化合物包括脂肪酸、脂肪醇、烷烃、甘油酯、α，ω-脂肪二酸和羟基脂肪酸。丰富的长链脂肪酸的非极性提取物和固体光滑的育空地区黄金品种建议广泛的木栓质生物聚合物的形成，这一假设是支持高比例的芳烃，烯烃和羰基在固体和极性标记。在非极性大西洋提取物和界面固体中没有许多潜在的标记类，表明木栓化程度有限。所有非极性提取物的适度清除活性表明，大多数的抗氧化剂产生的响应创伤是极性的。
The cultivation, storage, and distribution of potato tubers are compromised by mechanical damage and suboptimal healing. To investigate wound-healing progress in cultivars with contrasting russeting patterns, metabolite profiles reported previously for polar tissue extracts were complemented by GC/MS measurements for nonpolar extracts and quantitative 13C NMR of interfacial solid suspensions. Potential marker compounds that distinguish cultivar type and wound-healing time point included fatty acids, fatty alcohols, alkanes, glyceryl esters, α,ω-fatty diacids, and hydroxyfatty acids. The abundant long-chain fatty acids in nonpolar extracts and solids from the smooth-skinned Yukon Gold cultivar suggested extensive suberin biopolymer formation; this hypothesis was supported by high proportions of arenes, alkenes, and carbonyl groups in the solid and among the polar markers. The absence of many potential marker classes in nonpolar Atlantic extracts and interfacial solids suggested a limited extent of suberization. Modest scavenging activities of all nonpolar extracts indicate that the majority of antioxidants produced in response to wounding are polar.