Measurement of porcine luteinizing hormone concentration in blood by time-resolved fluoroimmunoassay
Measurement of porcine luteinizing hormone concentration in blood by time-resolved fluoroimmunoassay
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DOI：
10.1292/jvms.69.1291
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发表时间：
2007-12-01
影响因子：
1.2
通讯作者：
Itoh, Seigo
中科院分区：
文献类型：
--
作者：
Noguchi, Michiko;Yoshioka, Koji;Itoh, Seigo
我们验证了时间分辨荧光免疫分析法（TR-FIA），使用镧系元素铕作为非放射性示踪剂，用于测量猪外周血中的促黄体生成素（LH）。参比标准品的剂量-响应曲线范围为0.2048 - 50 ng/ml。LH标准品和血浆样品之间观察到良好的平行性。通过每日采血评估的整个发情周期的LH曲线与先前通过放射免疫测定（RIA）获得的结果一致。此外，在卵泡期和排卵前LH峰的脉冲LH的分泌模式也类似于以前的RIA中获得的。结论：TR-FIA可用于猪血液LH水平的测定，具有实用性和方便性。
We validated a time-resolved fluoroimmunoassay (TR-FIA) using the lanthanide element europium as a non-radiological tracer for measuring luteinizing hormone (LH) in porcine peripheral blood. The dose-response curve of the reference standard ranged from 0.2048 to 50 ng/ml. Good parallelism was noted between the LH standard and plasma sample. The profile of LH throughout the estrous cycle, assessed by daily blood sampling, was consistent with the previous findings obtained by radioimmunoassays (RIAs). Moreover, the secretary patterns of pulsatile LH in the follicular phase and the preovulatory LH surge were also similar to those obtained in previous RIAs. We conclude that TR-FIA can be used to measure LH levels in porcine blood, with practical and convenient applications.