Combinatorial effect of curcumin with docetaxel modulates apoptotic and cell survival molecules in prostate cancer.
Combinatorial effect of curcumin with docetaxel modulates apoptotic and cell survival molecules in prostate cancer.
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DOI：
10.2741/e798
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发表时间：
2017-03-01
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影响因子：
--
通讯作者：
Singh R
中科院分区：
文献类型：
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作者：
Banerjee S;Singh SK;Chowdhury I;Lillard JW Jr;Singh R
多西紫杉醇是转移性前列腺癌（PCa）中最常用的靶向雄激素信号的化疗药物；然而，长期使用多西紫杉醇治疗会导致耐药癌细胞。联合疗法有可能提高药物治疗的有效性，并减少副作用。姜黄素是一种无毒的有机化合物，具有多方面的化学预防潜力。在这项研究中，我们评估了姜黄素是否可以增强多西他赛对PCa细胞的作用。姜黄素和多西他赛分别单独或联合作用于前列腺癌细胞株DU145和PC3。处理完成后，分析细胞增殖和促生存、抗凋亡标志物及信号分子的表达情况。姜黄素和多西他赛联合治疗对细胞增殖的抑制作用和诱导的细胞凋亡明显高于姜黄素和多西他赛单独治疗组。有趣的是，姜黄素和多西紫杉醇联合治疗可调节rtk、PI3K、磷酸化akt、nf - κ B、p53和COX-2的表达。这些结果表明，姜黄素可能是一个潜在的治疗竞争者，以提高多西紫杉醇治疗前列腺癌的疗效。
Docetaxel is the most commonly used chemotherapeutic agent to target androgen signaling in metastatic prostate cancer (PCa); however, prolonged treatment with docetaxel results in drug-resistant cancer cells. Combination therapies have the potential of increasing the effectiveness of drug treatment as well as decreasing the side effects. Curcumin is a nontoxic organic compound with multifaceted chemopreventive potential. In this study, we evaluated whether curcumin can reinforce the effect of docetaxel on PCa cells. The PCa cell lines DU145 and PC3 were treated with curcumin and docetaxel alone or in combination. After completion of the treatment cell proliferation and the expression of pro-survival and anti-apoptotic markers and the signaling molecules were analyzed. The combined treatment of curcumin and docetaxel inhibited the proliferation and induced apoptosis significantly higher than the curcumin and docetaxel-treated group alone. Interestingly, the combined treatment with curcumin and docetaxel modulates the expression of RTKs, PI3K, phospho-AKT, NF-kappa B, p53, and COX-2. These results suggest that curcumin can be a potential therapeutic contender in enhancing the efficacy of docetaxel in PCa treatment.