Nonextractable polyphenols, usually ignored, are the major part of dietary polyphenols: A study on the Spanish diet
Nonextractable polyphenols, usually ignored, are the major part of dietary polyphenols: A study on the Spanish diet
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DOI：
10.1002/mnfr.200900580
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发表时间：
2010-11-01
影响因子：
5.2
通讯作者：
Saura-Calixto, Fulgencio
中科院分区：
文献类型：
--
作者：
Arranz, Sara;Manuel Silvan, Jose;Saura-Calixto, Fulgencio
范围：膳食多酚(PP)可分为两类：可提取多酚(EPP)或被水有机溶剂增溶的化合物，以及不可提取多酚(NEPP)或留在相应提取残留物中的化合物。大多数关于食物多酚和膳食摄入量的研究都只涉及EPP。本工作的目的是测定食品和整个饮食中PP的实际含量。方法和结果：利用高效液相-质谱仪分析了谷物、水果、蔬菜、坚果和豆类中甲醇-丙酮提取物中的EPP和其提取物残留物的酸性水解物中的NPP。NEPP的含量，即可水解性PP加上不可提取的原花青素(PA)，从水果的880 mg/100g干重到谷类的210 mg/100g干重不等，大大高于EPP的含量。西班牙人膳食中NEPP的摄入量(942 mg/人)高于EPP的摄入量(258 Mg)，水果和蔬菜(746 Mg)是PP总摄入量(1201 Mg)的主要贡献者。结论：膳食多酚中以不可提取的多酚为主。了解膳食PP的摄入量和生理特性有助于更好地了解膳食PP对健康的潜在影响。
Scope: Dietary polyphenols (PP) can be divided into two groups: extractable polyphenols (EPP) or compounds solubilized by aqueous organic solvents, and nonextractable polyphenols (NEPP) or compounds that remain in their corresponding extraction residues. Most studies on food polyphenols and dietary intakes address exclusively EPP. The objective of this work was to determine the actual amount of PP, including NEPP, in food and in a whole diet.Methods and results: HPLC-MS analyses were performed to identify EPP in methanol-acetone extracts and NEPP in the acidic hydrolyzates of their extraction residues in cereals, fruits, vegetables, nuts, and legumes. NEPP contents, estimated as hydrolyzable PP plus nonextractable proanthocyanidins (PA), ranged from 880 mg/100 g dry weight in fruits to 210 mg/100 g in cereals and were substantially higher than the contents of EPP. NEPP intake (day/person) in the Spanish diet (942 mg) is higher than EPP intake (258 mg) fruits and vegetables (746 mg) are the major contributors to the total PP intake (1201 mg).Conclusion: Non extractable polyphenols are the major part of dietary polyphenols. The knowledge of intakes and physiological properties of NEPP may be useful for a better understanding of the potential health effects of dietary PP.