Tumor-educated platelet SNORD55 as a potential biomarker for the early diagnosis of non-small cell lung cancer.
Tumor-educated platelet SNORD55 as a potential biomarker for the early diagnosis of non-small cell lung cancer.
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血小板SNORD55作为非小细胞肺癌早期诊断的潜在生物标志物
DOI：
10.1111/1759-7714.13823
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发表时间：
2021-03
期刊：
影响因子：
2.9
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Dong X;Song X;Ding S;Yu M;Shang X;Wang K;Chang M;Xie L;Song X
尽管许多snoRNAs(小核仁RNAs)可以在体液中检测到并作为非侵入性生物标志物，但以前很少有研究讨论snoRNAs在肿瘤教育血小板(TEP)中的作用。在此，我们系统地评估了非小细胞肺癌(NSCLC)中snoRNAs的异常调节，并阐明了SNORD55在血小板中的生物标志物潜力。我们使用SNORic数据集比较了非小细胞肺癌和正常组织中snoRNAs的表达。采用低速离心法从血浆中分离血小板，用定量聚合酶链式反应(QPCR)检测SNORD55。NSCLC患者TEP中SNORD55较正常对照组明显降低，尤其是早期患者。重要的是，我们验证了TEP SNORD55能够作为NSCLC的一个有前途的生物标记物。对非小细胞肺癌的诊断，AUC值为0.803；对非小细胞肺癌的早期诊断，AUC值为0.784。此外，TEP SNORD55和癌胚抗原(CEA)的结合提高了对癌症进展的诊断效率。此外，TEP SNORD55还可能作为肺腺癌(LUAD)和肺鳞癌(LUSC)的非侵袭性早期生物标志物，具有良好的诊断效率。综上所述，TEP SNORD55有可能作为非小细胞肺癌诊断和早期诊断的无创性生物标志物。NSCLC患者TEP中SNORD55显著低于健康对照组，可作为早期NSCLC的一个新的生物标志物。我们的研究结果揭示了NSCLC患者血小板中SNORD55的肿瘤特异性变化，为单独或联合开发敏感、特异的NSCLC诊断标志物铺平了道路，为TEP SNORD55在NSCLC早期筛查中的临床项目提供了一定的依据。
Despite the emerging insights into many snoRNAs (small nucleolar RNAs) which are detectable in body fluids and serve as noninvasive biomarkers, few studies have previously discussed the role of snoRNAs in tumor‐educated platelets (TEPs). Herein, we systematically estimated dysregulation of snoRNAs in non‐small cell lung cancer (NSCLC) and clarified the biomarker potential of SNORD55 in platelets. We compared expression of snoRNAs between NSCLC and normal tissues using SNORic datasets. Platelets were isolated from plasma using low‐speed centrifugation and subjected to quantitative polymerase chain reaction (qPCR) for SNORD55 detection. SNORD55 was significantly decreased in TEPs from NSCLC patients especially in early‐stage patients compared with healthy controls. Importantly, we validated that TEP SNORD55 was capable of acting as a promising biomarker for NSCLC. It exerted diagnostic performance for NSCLC diagnosis, possessing an AUC of 0.803, as well as for early NSCLC diagnosis, possessing an AUC of 0.784. Moreover, the combination of TEP SNORD55 and carcinoembryonic antigen (CEA) improved the diagnostic efficiency of cancer progression. In addition, TEP SNORD55 also potentially acts as a noninvasive early biomarker for lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) with favorable diagnostic efficiencies. In summary, TEP SNORD55 could potentially serve as a noninvasive biomarker for NSCLC diagnosis and early diagnosis. SNORD55 was significantly decreased in TEPs from NSCLC patients compared to healthy controls and acted as a novel biomarker for early NSCLC. Our findings reveal cancer‐specific changes of SNORD55 in platelets of NSCLC, which paving the way for the exploitation of sensitive and specific NSCLC diagnostic biomarker either alone or in combination, providing certain basis for clinical projects of TEP SNORD55 in early screening of NSCLC.
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