BENEFICIAL INFLUENCE OF GLYCEMIC CONTROL UPON THE GROWTH AND FUNCTION OF TRANSPLANTED ISLETS
BENEFICIAL INFLUENCE OF GLYCEMIC CONTROL UPON THE GROWTH AND FUNCTION OF TRANSPLANTED ISLETS
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DOI：
10.2337/diabetes.43.11.1334
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发表时间：
1994-11-01
期刊：
影响因子：
7.7
通讯作者：
WEIR, GC
中科院分区：
文献类型：
--
作者：
JUANG, JH;BONNERWEIR, S;WEIR, GC
在肾囊下移植 200 个小鼠胰岛通常无法治愈链佐星诱发的糖尿病。我们假设，如果将这些数量的胰岛移植到血糖正常的环境中，它们可以扩大其质量并改善其功能以恢复正常血糖。因此，将200个新鲜分离的小鼠胰岛移植到糖尿病小鼠每只肾的被膜下。移植后两周，受体血糖正常，两个移植物之一被移除。移除移植物后出现短暂的高血糖。移植物移除后第 14 天，剩余移植物的 β 细胞质量和胰岛素含量分别增加了 2.3 倍和 2.1 倍。移植物去除后第 3 天，β 细胞的复制率增加了三倍，并且平均个体 β 细胞横截面积（细胞大小的指标）也增加了。移除一颗移植物后 14 天，带有剩余移植物的肾脏的灌注显示出对高葡萄糖和精氨酸的双相胰岛素反应。这些数据表明，胰岛移植后维持一段接近正常血糖的时期可以增强胰岛移植物的性能，否则胰岛移植物预计会失败。
Syngeneic transplantation of 200 mouse islets under the kidney capsule usually fails to cure streptozocin-induced diabetes. We hypothesized that this number of islets, if engrafted in a normoglycemic environment, could expand their mass and improve their function to restore normoglycemia. Therefore, 200 freshly isolated mouse islets were transplanted under the capsule of each kidney of diabetic mice. Two weeks after transplantation, the recipients were normoglycemic, and one of the two grafts was removed. Removal of the graft was followed by transient hyperglycemia. At day 14 after graft removal, the beta-cell mass and insulin content of the remaining graft had increased 2.3- and 2.1-fold, respectively. At day 3 after graft removal, the replication rate of beta-cells increased threefold, and the mean individual beta-cell cross-sectional area, an indicator of cell size, was also increased. Perfusion of the kidney bearing the remaining graft showed biphasic insulin responses to high glucose and arginine 14 days after one graft removal. These data indicate that maintaining a period of near-normoglycemia after islet transplantation enhances the performance of an islet graft that would otherwise be expected to fail.