Effects of Lactobacillus acidophilus NCFM on insulin sensitivity and the systemic inflammatory response in human subjects
Effects of Lactobacillus acidophilus NCFM on insulin sensitivity and the systemic inflammatory response in human subjects
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DOI：
10.1017/s0007114510002874
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发表时间：
2010-12-01
影响因子：
3.6
通讯作者：
Pedersen, Bente Klarlund
中科院分区：
文献类型：
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作者：
Andreasen, Anne Sofie;Larsen, Nadja;Pedersen, Bente Klarlund
根据动物研究，摄入益生菌可能会改善血糖稳态。我们假设益生菌通过减轻全身炎症来改善胰岛素敏感性。因此，我们研究了口服益生菌嗜酸乳杆菌NCFM对胰岛素敏感性和胰岛素敏感性受损受试者炎症反应的影响。采用双盲随机方法，将45名患有2型糖尿病、糖耐量受损或正常的男性患者分配给嗜酸乳杆菌NCFM或安慰剂进行为期4周的治疗。用变性梯度凝胶电泳法和实时荧光定量聚合酶链式反应检测粪便标本中的嗜酸乳杆菌。以4周的干预期分开，进行两次高胰岛素-正常血糖钳夹以评估胰岛素敏感性。此外，通过在疗程前和疗程后给予大肠杆菌脂多糖注射(0.3 ng/kg)来评估全身炎症反应。经益生菌处理后，75%的粪样中检出嗜酸乳杆菌NCFM。嗜酸乳杆菌NCFM组志愿者的胰岛素敏感性得以保存，而安慰剂组的胰岛素敏感性有所降低。然而，基线炎症标志物和全身炎症反应均未受到干预的影响。总之，与安慰剂相比，摄入4周的嗜酸乳杆菌NCFM保持了胰岛素敏感性，但不影响全身炎症反应。
According to animal studies, intake of probiotic bacteria may improve glucose homeostasis. We hypothesised that probiotic bacteria improve insulin sensitivity by attenuating systemic inflammation. Therefore, the effects of oral supplementation with the probiotic bacterium Lactobacillus acidophilus NCFM on insulin sensitivity and the inflammatory response were investigated in subjects with normal or impaired insulin sensitivity. In a double-blinded, randomised fashion, forty-five males with type 2 diabetes, impaired or normal glucose tolerance were enrolled and allocated to a 4-week treatment course with either L. acidophilus NCFM or placebo. L. acidophilus was detected in stool samples by denaturating gradient gel electrophoresis and real-time PCR. Separated by the 4-week intervention period, two hyperinsulinaemic-euglycaemic clamps were performed to estimate insulin sensitivity. Furthermore, the systemic inflammatory response was evaluated by subjecting the participants to Escherichia coli lipopolysaccharide injection (0.3 ng/kg) before and after the treatment course. L. acidophilus NCFM was detected in 75% of the faecal samples after treatment with the probiotic bacterium. Insulin sensitivity was preserved among volunteers in the L. acidophilus NCFM group, whereas it decreased in the placebo group. Both baseline inflammatory markers and the systemic inflammatory response were, however, unaffected by the intervention. In conclusion, intake of L. acidophilus NCFM for 4 weeks preserved insulin sensitivity compared with placebo, but did not affect the systemic inflammatory response.