CD24 promotes HCC progression via triggering Notch-related EMT and modulation of tumor microenvironment
CD24 promotes HCC progression via triggering Notch-related EMT and modulation of tumor microenvironment
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DOI：
10.1007/s13277-015-4442-7
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发表时间：
2016-05-01
期刊：
影响因子：
--
通讯作者：
Chen, Yun
中科院分区：
文献类型：
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作者：
Wan, Xin;Cheng, Ci;Chen, Yun
CD24 被称为造血细胞表面分子，也被描述为肿瘤的诊断标志物。先前的研究表明CD24在肝细胞癌（HCC）发病机制中发挥重要作用。然而，CD24 在 HCC 中的精确功能仍不清楚。在这里，我们发现 CD24 在 HCC 中的 mRNA 和蛋白质水平均高表达。此外，CD24 介导的上皮间质转化 (EMT) 和 Notch1 信号激活被阐明为 Hepa1-6/Hepa1-6-CD24 细胞模型中促进 HCC 的潜在机制。此外，通过免疫细胞和Hepa1-6/Hepa1-6-CD24细胞共培养探索了可能的全身免疫反应。我们证明CD24能有效诱导HCC细胞的EMT过程；此外，通过促进体内Notch相关EMT改变肿瘤免疫微环境。这些结果揭示了 CD24 介导的 HCC 过程之间的潜在联系。此外，作为一种明确的肿瘤促进剂，CD24被认为是HCC治疗的潜在新靶点。
CD24 is known as a cell surface molecule in hematopoiesis and also described as a diagnostic marker for tumors. Previous studies suggested the important role of CD24 in hepatocellular carcinoma (HCC) pathogenesis. However, precise functions of CD24 in HCC are still unknown. Here, we found that CD24 is highly expressed in HCC both in mRNA and protein levels. Further, the epithelial-mesenchymal transition (EMT) and Notch1 signaling activations mediated by CD24 were elucidated as potential mechanisms of HCC promotion in Hepa1-6/ Hepa1-6-CD24 cell models. Additionally, possible systemic immune reaction was explored through immune cells and Hepa1-6/ Hepa1-6-CD24 cell co-culture. We demonstrated that the EMT process of HCC cell was effectively induced by CD24; also, the tumor immune microenvironment was changed by facilitating Notch-related EMT in vivo. These results reveal the underlying link between the HCC processes mediated by CD24. Moreover, as a clear tumor promoter, CD24 is considered a potential new target for HCC treatment.