Perturbing Tandem Energy Transfer in Luminescent Heterobinuclear Lanthanide Coordination Polymer Nanoparticles Enables Real-Time Monitoring of Release of the Anthrax Biomarker from Bacterial Spores
Perturbing Tandem Energy Transfer in Luminescent Heterobinuclear Lanthanide Coordination Polymer Nanoparticles Enables Real-Time Monitoring of Release of the Anthrax Biomarker from Bacterial Spores
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发光异双核镧系元素配位聚合物纳米颗粒中的扰动串联能量转移能够实时监测细菌孢子中炭疽生物标志物的释放
DOI：
10.1021/acs.analchem.8b01365
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发表时间：
2018-06-05
影响因子：
7.4
通讯作者：
Mao, Lanqun
中科院分区：
文献类型：
--
作者：
Gao, Nan;Zhang, Yunfang;Mao, Lanqun
基于镧系元素的发光传感器已广泛用于炭疽生物标志物吡啶二甲酸（DPA）的检测。然而，主要基于DPA对镧系核心的敏化，大多数未能实现细菌孢子中DPA的稳健检测。我们提出了一种可靠检测 DPA 的新策略，通过干扰异双核稀土配位聚合物纳米颗粒中的串联能量转移，该纳米颗粒由两种稀土离子 Tb3+ 和 Eu3+ 以及鸟苷 5'-单磷酸简单构建。事实证明，这种智能发光探针在接触 DPA 时表现出高度灵敏和选择性的视觉发光颜色变化，从而能够准确检测复杂生物系统（例如细菌孢子）中的 DPA。通过共焦成像也成功地实时监测了生理萌发时细菌孢子释放的 DPA。因此，该探针有望成为有效检测细菌孢子以应对炭疽威胁的强大工具。
Lanthanide-based luminescent sensors have been widely used for the detection of the anthrax biomarker dipicolinic acid (DPA). However, mainly based on DPA sensitization to the lanthanide core, most of them failed to realize robust detection of DPA in bacterial spores. We proposed a new strategy for reliable detection of DPA by perturbing a tandem energy transfer in heterobinuclear lanthanide coordination polymer nanoparticles simply constructed by two kinds of lanthanide ions, Tb3+ and Eu3+, and guanosine 5'-monophosphate. This smart luminescent probe was demonstrated to exhibit highly sensitive and selective visual luminescence color change upon exposure to DPA, enabling accurate detection of DPA in complex biosystems such as bacterial spores. DPA release from bacterial spores on physiological germination was also successfully monitored in real time by confocal imaging. This probe is thus expected to be a powerful tool for efficient detection of bacterial spores in responding to anthrax threats.