A partially purified glycoprotein antigen from Aspergillus fumigatus.
A partially purified glycoprotein antigen from Aspergillus fumigatus.
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来自烟曲霉的部分纯化的糖蛋白抗原。
DOI：
10.1159/000233984
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Fink,JN
中科院分区：
文献类型：
--
作者：
Kurup,VP;John,KV;Resnick,A;Fink,JN
用等电聚焦法，用4-6.5的pH梯度，对烟曲霉培养滤液抗原进行分级。将三个级分，即18、19和20合并并进行免疫化学分析。它含有伴刀豆球蛋白A结合糖蛋白。针对该组分产生的抗体用于制备IgG-琼脂糖凝胶亲和柱，并用于纯化粗培养滤液抗原。该组分在使用抗A的交叉免疫电泳中产生三个沉淀素弧。熏兔血清该组分的等电点为6.5，在二维电泳中显示出三种组分，分子量约为20、40和80千道尔顿。该抗原在生物素亲和素连接免疫吸附试验中与患者血清反应，并显示高水平的抗A。过敏性支气管肺曲霉病IgG和IgE抗体和曲霉瘤IgG抗体。对照组和豚鼠皮肤试验阳性的哮喘患者均显示低水平的特异性抗体。由于该抗原的纯度，其潜在的使用作为一个标准化的抗原在抗体的检测进行了讨论。
Culture filtrate antigens ofAspergillus fumigatuswere fractionated by isoelectric focusing using a pH gradient of 4–6.5. Three fractions, namely, 18, 19 and 20 were pooled and subjected to immunochemical analysis. It contained a concanavalin A binding glycoprotein. Antibody raised against this component was used to prepare an affinity column of IgG-Sepharose and was used to purify crude culture filtrate antigens. This component produced three precipitin arcs in crossed immunoelectrophoresis using anti-A. fumigatusrabbit serum. This fraction has an isoelectric point of 6.5 and showed three components in two-dimensional electrophoresis with approximate molecular weights of 20, 40 and 80 kilo daltons. This antigen reacted with patient sera in the biotinavidin linked immunosorbent assay and showed high levels of anti-A. fumigatusIgG and IgE antibodies in allergic bronchopulmonary aspergillosis and IgG antibodies in aspergilloma. Both controls andAspergillusskin test positive asthmatics showed only low levels of specific antibodies. Because of the purity of this antigen, its potential use as a standardized antigen in the detection of antibody is discussed.