Irradiation of solutions of transforming DNA.
Irradiation of solutions of transforming DNA.
复制标题
照射转化 DNA 的溶液。
DOI：
10.2307/3570732
复制
发表时间：
1959
影响因子：
3.4
通讯作者：
W. R. Guild
中科院分区：
文献类型：
--
作者：
F. Defilippes;W. R. Guild
在多种浓度的溶液中对转化DNA进行了辐射，并测定了使生物转化活性失活的离子产额。D37值的范围从65伦琴到数千伦琴，这取决于DNA的制备方法和浓度。观察到的最大产额表明，每个离子对可使分子量为500,000的物质失活，这与直接作用所观察到的敏感性也相当接近。未观察到辐射的后效应，也未发现去除O₂的影响。似乎在足够纯的溶液中，可以观察到至少两种中间产物（疑似为OH和H）的影响。最后，可以得出结论：在体外，如果一个自由基能够到达DNA分子，它使DNA分子生物失活的概率接近1，但在体内，产生的自由基中可能只有相对较少的一部分能够找到DNA。
Transforming DNA has been irradiated in solution over a wide range of concentrations, and the ionic yield determined for inactivation of the biological transforming activity. The D37's ranged from 65 r to several thousand roentgens, depending on the DNA preparation and the concentration. The maximum observed yield indicated that a mass of 500,000 molecular weight was inactivated per ion pair, which corresponds rather closely to the sensitivity observed for direct action also. No aftereffect of irradiation was observed, nor was an effect of removing O2 found. It appears that in sufficiently pure solutions one may observe the effect of at least two intermediates, suspected as being OH and H. Finally, it may be concluded that in vitro a radical has a probability near unity of inactivating a DNA molecule biologically if it gets to the molecule, but that in vivo relatively few of the radicals produced probably do find the DNA.