Optical Clearing of Vaginal Tissues in Cadavers.
Optical Clearing of Vaginal Tissues in Cadavers.
复制标题
尸体阴道组织的光学清除。
DOI：
10.1117/12.2285079
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fried,NathanielM
中科院分区：
文献类型：
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作者：
Chang,Chun-Hung;Hardy,LukeA;Peters,MichaelG;Bastawros,DinaA;Myers,ErinnM;Kennelly,MichaelJ;Fried,NathanielM
一种非手术激光手术正在开发用于治疗女性压力性尿失禁（SUI）。先前在猪阴道组织中进行的离体研究以及计算机模拟显示了使用通过经阴道接触冷却探针递送的近红外激光能量来热重塑骨盆内筋膜的可行性，同时保护阴道壁免受热损伤。本研究探讨了尸体阴道组织的光学特性，作为未来临床前和临床研究的中间步骤。在室温下11分钟后，使用甘油对组织进行光学清洁导致光学透射率增加15-17%（在体温下计算增加32.5%）。使用4.6 - 6.4 W的功率、5.2 mm的光斑和30 s的照射时间产生皮下热损伤，导致阴道壁部分保留至0.8 - 1.1 mm深度。
A nonsurgical laser procedure is being developed for treatment of female stress urinary incontinence (SUI). Previous studies in porcine vaginal tissues, ex vivo, as well as computer simulations, showed the feasibility of using near-infrared laser energy delivered through a transvaginal contact cooling probe to thermally remodel endopelvic fascia, while preserving the vaginal wall from thermal damage. This study explores optical properties of vaginal tissue in cadavers as an intermediate step towards future pre-clinical and clinical studies. Optical clearing of tissue using glycerol resulted in a 15-17% increase in optical transmission after 11 min at room temperature (and a calculated 32.5% increase at body temperature). Subsurface thermal lesions were created using power of 4.6 - 6.4 W, 5.2-mm spot, and 30 s irradiation time, resulting in partial preservation of vaginal wall to 0.8 - 1.1 mm depth.