STRUCTURAL RELATIONSHIPS BETWEEN CLATHRIN ASSEMBLY PROTEINS FROM THE GOLGI AND THE PLASMA-MEMBRANE
STRUCTURAL RELATIONSHIPS BETWEEN CLATHRIN ASSEMBLY PROTEINS FROM THE GOLGI AND THE PLASMA-MEMBRANE
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DOI：
10.1002/j.1460-2075.1988.tb02897.x
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发表时间：
1988-04-01
期刊：
影响因子：
11.4
通讯作者：
UNGEWICKELL, E
中科院分区：
文献类型：
--
作者：
AHLE, S;MANN, A;UNGEWICKELL, E
我们通过肽图谱和免疫化学分析，对纯化的牛脑网格蛋白组装蛋白制备物进行了研究。WT 100-120 kD分为四类，我们称之为α，β，β。和γ..的β和β蛋白质在免疫学上是相关的，并产生一系列常见的胰蛋白酶肽。相同的标准揭示了α，β。（β）“）和γ。多肽。所谓的HA-II装配蛋白质组含有等摩尔量的α。和β类多肽，其显示彼此相互作用。在HA-I组装配蛋白复合物γ中，和β"类多肽形成化学计量复合物。免疫荧光显微镜显示，HA-I复合物是专门与网格蛋白包被的膜在高尔基体区域的培养细胞，而HA-II复合物似乎被限制在质膜上的涂层坑。这些数据导致的初步结论，网格蛋白组装蛋白参与识别的细胞内目标的未涂层囊泡。
We have established by peptide mapping and immunochemical analysis of purified clathrin assembly protein preparations for bovine brain, that the cluster of components of mol. wt 100-120 kd fall into four classes, which we term .alpha., .beta., .beta.'' and .gamma.. The .beta. and .beta.'' proteins are immunologically related and generate a series of common tryptic peptides. The same criteria reveal no such homologies between the .alpha., .beta.(.beta.'') and .gamma. polypeptides. The so-called HA-II assembly protein group contains equimolar amounts of .alpha. and .beta. class polypeptides, which are shown to interact with each other. In the HA-I group assembly protein complex .gamma. and .beta.'' class polypeptides form a stoichiometric complex. Immunofluorescence microscopy reveals that the HA-I complex is specifically associated with clathrin-coated membranes in the Golgi region of cultured cells, whereas the HA-II complex appears to be restricted to coated pits on the plasma membrane. The data lead to the tentative conclusion that the clathrin assembly proteins are involved in the recognition of the intracellular targets by uncoated vesicles.