Effects of α-TCP and TetCP on MC3T3-E1 proliferation, differentiation and mineralization
Effects of α-TCP and TetCP on MC3T3-E1 proliferation, differentiation and mineralization
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DOI：
10.1016/s0142-9612(02)00411-8
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发表时间：
2003-02-01
期刊：
影响因子：
14
通讯作者：
Umakoshi, Y
中科院分区：
文献类型：
--
作者：
Ehara, A;Ogata, K;Umakoshi, Y
在水中具有高溶解度的磷酸钙，如α-磷酸三钙（α-TCP）和磷酸四钙（TetCP），作为骨替代材料的组分受到了相当大的关注。然而，成骨细胞对这些材料的反应还没有被清楚地了解。本研究探讨了α-TCP和TetCP对MC 3 T3-E1细胞培养体系中成骨细胞增殖、分化和矿化的影响。在含有或不含有1或10 μ m α-TCP或TetCP的分化培养基中培养细胞，并测定附着于培养板的细胞数。检测成骨细胞分化。检测碱性磷酸酶（ALP）活性，RT-PCR检测成骨细胞标志物的表达。此外，通过用茜素红对钙存款进行染色来评价矿化。在α-TCP或TetCP存在下的培养物显示对细胞增殖没有显著影响。两种磷酸钙均能促进细胞ALP活性，并在培养后12 h和1 d促进1型胶原的表达。通过添加10 μ m的α-TCP或TetCP，在7天孵育后及其后观察到骨样组织形成的增强。本研究的结果表明，α-TCP和TetCP通过增加胶原蛋白合成和细胞外基质钙化来促进骨生成。(C)2002爱思唯尔科技有限公司版权所有。
Calcium phosphates with high solubility in water such as alpha-tricalcium phosphate (alpha-TCP) and tetracalcium phosphate (TetCP) have received considerable attention as components of bone-substitution Materials. However, the osteoblast response to these materials has not yet been clearly understood. This study examined the effects of alpha-TCP and TetCP on osteoblast proliferation, differentiation and mineralization in the culture system of MC3T3-E1 cells. Cells were Cultured in a differentiation medium with or without alpha-TCP or TetCP at 1 or 10 mum, and the number of cells attached to the culture plates was determined. To examine osteoblast differentiation. the alkaline phosphatase (ALP) activity was measured and the expression of osteoblastic markers analyzed by RTPCR. In addition, mineralization was evaluated by staining the calcium deposit with Alizalin red. Culture in the presence of alpha-TCP or TetCP showed no significant influence on cell proliferation. ALP activities of the cells were enhanced by both calcium phosphates for 3 d and the expression of type 1 collagen was promoted at 12 h and 1 d after incubation. Enhancement of bone-like tissue formation by the addition of alpha-TCP or TetCP at 10 mum was observed after 7 d incubation and thereafter. The results of the present study indicate that alpha-TCP and TetCP promote osteogenesis by increasing collagen synthesis and calcification of the extra-cellular matrix. (C) 2002 Elsevier Science Ltd. All rights reserved.